毛细管区带电泳技术研究龙血竭有效单体与核酸适配体及凝血酶之间的相互作用

Translated title of the contribution: Interactions Between Aptamer, Bovine Thrombin and Active Molecules in Dragon's Blood by CZE

Yu Juan Li, Xin Xin Sun*, Pan Pan Li, Wan Lu Ou, Feng Qu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Interactions between Apt29, bovine thrombin (B-Thr) and five active molecules in Dragon's blood were investigated based on capillary zone electrophoresis (CZE). The CZE conditions were set as following: effective capillary length of 40 cm, injection volume of 3.45 kPa, injection time of 5 s, voltage of +15 kV, temperature of 15℃, detection wavelength of 214 nm and 330 nm. The results show that Kb between Loureirin A and B-Thr is 4.73×104 L/mol, and Loureirin B, Loureirin C, Resveratrol 7, 4-dihydroxyflavanone are no bound with B-Thr obviously. The Kb between Loureirin B, DHF and Apt29 are 1.98×104, 1.83×104 L/mol, respectively. Loureirin A, Loureirin C and Resveratrol are no bound with Apt29 obviously. The study indicates the interactions between B-Thr, Apt29 and active molecules in Dragon's blood. It can provide a basis for speculating the potential binding site of dragon's blood with thrombin, and delivering Dragon's blood to thrombin with aptamer as target delivery tool.

Translated title of the contributionInteractions Between Aptamer, Bovine Thrombin and Active Molecules in Dragon's Blood by CZE
Original languageChinese (Traditional)
Pages (from-to)106-110
Number of pages5
JournalBeijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume39
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

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