TY - JOUR
T1 - Vitellogenic effects of 17β-estradiol in male Chinese loach (Misgurnus anguillicaudatus)
AU - Lv, Xuefei
AU - Shao, Jing
AU - Song, Maoyong
AU - Zhou, Qunfang
AU - Jiang, Guibin
PY - 2006/5
Y1 - 2006/5
N2 - Male Chinese loaches (Misgurnus anguillicaudatus) were exposed to 17β-estradiol (E2) in the ambient water at nominal concentrations of 0.5, 1, 5 and 10 μg/L continuously for 8 weeks using a semi-static system. The gonadosomatic index (GSI), total plasma protein, total plasma calcium, magnesium and zinc, and plasma vitellogenin (Vtg) were determined as test endpoints. Our results indicate no significant changes in the GSI between exposed and control groups (P < 0.05). All exposure concentrations of E2 dramatically induced the production of Vtg within 7 days. The vitellogenic response of male loach was time- and dose-dependent. At higher concentrations of E2 (5 and 10 μg/L), total plasma protein, total plasma calcium, and total plasma magnesium presented a significant time- and dose-dependent increase, and the three parameters were significantly correlated with plasma Vtg. We conclude that Chinese loaches are sensitive to estrogenic compounds and may be chosen as potential sentinel species in field and laboratory studies. In addition, total plasma protein, total plasma calcium, and total plasma magnesium can be used as indirect indicators to predict Vtg levels in estrogenized Chinese loach.
AB - Male Chinese loaches (Misgurnus anguillicaudatus) were exposed to 17β-estradiol (E2) in the ambient water at nominal concentrations of 0.5, 1, 5 and 10 μg/L continuously for 8 weeks using a semi-static system. The gonadosomatic index (GSI), total plasma protein, total plasma calcium, magnesium and zinc, and plasma vitellogenin (Vtg) were determined as test endpoints. Our results indicate no significant changes in the GSI between exposed and control groups (P < 0.05). All exposure concentrations of E2 dramatically induced the production of Vtg within 7 days. The vitellogenic response of male loach was time- and dose-dependent. At higher concentrations of E2 (5 and 10 μg/L), total plasma protein, total plasma calcium, and total plasma magnesium presented a significant time- and dose-dependent increase, and the three parameters were significantly correlated with plasma Vtg. We conclude that Chinese loaches are sensitive to estrogenic compounds and may be chosen as potential sentinel species in field and laboratory studies. In addition, total plasma protein, total plasma calcium, and total plasma magnesium can be used as indirect indicators to predict Vtg levels in estrogenized Chinese loach.
KW - Calcium
KW - Chinese loach
KW - Experimental animal
KW - Indirect indicator
KW - Magnesium
KW - Misgurnus anguillicaudatus
KW - Total plasma protein
KW - Vitellogenin
UR - http://www.scopus.com/inward/record.url?scp=33645392503&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2006.01.002
DO - 10.1016/j.cbpc.2006.01.002
M3 - Article
C2 - 16490402
AN - SCOPUS:33645392503
SN - 1532-0456
VL - 143
SP - 127
EP - 133
JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
IS - 1
ER -