The investigation of dynamically immobilized MAO on IAM using biologically functionalized chromatography

Yueying Liu, Rong Ji Dai, Feng Qu, Weiwei Meng, Yulin Deng*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

In this paper, the retention times of Serotonin (5-HT) were investigated on IAM (immobilized artificial membrane) column and IAM-MAO column which MAO was inhibited by clorgyline at various concentrations. The retention time of 5-HT on IAM column was shorter than that on IAM-MAO column. With the increasing of inhibitor concentrations, the retention time of 5-HT on IAM-MAO column became shorter and shorter. The results demonstrated that the MAO was successfully immobilized on IAM. The dynamical immobilized MAO retained its bioactivity. The establishment of artificial cell could better simulate the interaction between 5-HT and MAO in organism. Thus this study proved that BioFC (biologically functionalized chromatography) might become a new concept and method to simulate cell (artificial cell) and a possible tool for the drug screening in the future.

源语言英语
主期刊名2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
1787-1791
页数5
DOI
出版状态已出版 - 2007
活动2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, 中国
期限: 23 5月 200727 5月 2007

出版系列

姓名2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

会议

会议2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
国家/地区中国
Beijing
时期23/05/0727/05/07

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