Zhang, P., Chen, Y., Zhu, H., Yan, L., Sun, C., Pei, S., Lodhi, A. F., Ren, H., Gao, Y., Manzoor, R., Li, B., Deng, Y., & Ma, H. (2019). The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An in Vitro and in Vivo Study. Radiation Research, 192(4), 440-450. https://doi.org/10.1667/RR15378.1
Zhang, Peng ; Chen, Yu ; Zhu, Huiyang 等. / The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response : An in Vitro and in Vivo Study. 在: Radiation Research. 2019 ; 卷 192, 号码 4. 页码 440-450.
@article{5241c9a6e3964b49b060a489a93a8617,
title = "The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An in Vitro and in Vivo Study",
abstract = "Radiotherapy to treat brain tumors can potentially harm the central nervous system (CNS). The radiation stimulates a series of immune responses in both the CNS as well as peripheral immune system. To date, studies have mostly focused on the changes occurring in the immune response within the CNS. In this study, we investigated the effect of ?-ray-induced CNS injury on the peripheral immune response using a cell co-culture model and a whole-brain irradiation (WBI) rat model. Nerve cells (SH-SY5Y and U87 MG cells) were ?-ray irradiated, then culture media of the irradiated cells (conditioned media) was used to culture immune cells (THP-1 cells or Jurkat cells). Analyses were performed based on the response of immune cells in conditioned media. Sprague-Dawley rats received WBI at different doses, and were fed for one week to one month postirradiation. Spleen and peripheral blood were then isolated and analyzed. We observed that the number of monocytes in peripheral blood, and the level of NK cells and NKT cells in spleen increased after CNS injury. However, the level of T cells in spleen did not change and the level of B cells in the spleen decreased after ?-ray-induced CNS injury. These findings indicate that CNS injury caused by ionizing radiation induces a series of changes in the peripheral immune system.",
author = "Peng Zhang and Yu Chen and Huiyang Zhu and Liben Yan and Chunli Sun and Sizhu Pei and Lodhi, {Adil Farooq} and Hao Ren and Yanan Gao and Robina Manzoor and Bo Li and Yulin Deng and Hong Ma",
note = "Publisher Copyright: {\textcopyright} 2019 by Radiation Research Society. All rights of reproduction in any form reserved.",
year = "2019",
month = oct,
day = "1",
doi = "10.1667/RR15378.1",
language = "English",
volume = "192",
pages = "440--450",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "Radiation Research Society",
number = "4",
}
Zhang, P, Chen, Y, Zhu, H, Yan, L, Sun, C, Pei, S, Lodhi, AF, Ren, H, Gao, Y, Manzoor, R, Li, B, Deng, Y & Ma, H 2019, 'The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An in Vitro and in Vivo Study', Radiation Research, 卷 192, 号码 4, 页码 440-450. https://doi.org/10.1667/RR15378.1
The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An in Vitro and in Vivo Study. / Zhang, Peng; Chen, Yu; Zhu, Huiyang 等.
在:
Radiation Research, 卷 192, 号码 4, 01.10.2019, 页码 440-450.
科研成果: 期刊稿件 › 文章 › 同行评审
TY - JOUR
T1 - The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response
T2 - An in Vitro and in Vivo Study
AU - Zhang, Peng
AU - Chen, Yu
AU - Zhu, Huiyang
AU - Yan, Liben
AU - Sun, Chunli
AU - Pei, Sizhu
AU - Lodhi, Adil Farooq
AU - Ren, Hao
AU - Gao, Yanan
AU - Manzoor, Robina
AU - Li, Bo
AU - Deng, Yulin
AU - Ma, Hong
N1 - Publisher Copyright:
© 2019 by Radiation Research Society. All rights of reproduction in any form reserved.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Radiotherapy to treat brain tumors can potentially harm the central nervous system (CNS). The radiation stimulates a series of immune responses in both the CNS as well as peripheral immune system. To date, studies have mostly focused on the changes occurring in the immune response within the CNS. In this study, we investigated the effect of ?-ray-induced CNS injury on the peripheral immune response using a cell co-culture model and a whole-brain irradiation (WBI) rat model. Nerve cells (SH-SY5Y and U87 MG cells) were ?-ray irradiated, then culture media of the irradiated cells (conditioned media) was used to culture immune cells (THP-1 cells or Jurkat cells). Analyses were performed based on the response of immune cells in conditioned media. Sprague-Dawley rats received WBI at different doses, and were fed for one week to one month postirradiation. Spleen and peripheral blood were then isolated and analyzed. We observed that the number of monocytes in peripheral blood, and the level of NK cells and NKT cells in spleen increased after CNS injury. However, the level of T cells in spleen did not change and the level of B cells in the spleen decreased after ?-ray-induced CNS injury. These findings indicate that CNS injury caused by ionizing radiation induces a series of changes in the peripheral immune system.
AB - Radiotherapy to treat brain tumors can potentially harm the central nervous system (CNS). The radiation stimulates a series of immune responses in both the CNS as well as peripheral immune system. To date, studies have mostly focused on the changes occurring in the immune response within the CNS. In this study, we investigated the effect of ?-ray-induced CNS injury on the peripheral immune response using a cell co-culture model and a whole-brain irradiation (WBI) rat model. Nerve cells (SH-SY5Y and U87 MG cells) were ?-ray irradiated, then culture media of the irradiated cells (conditioned media) was used to culture immune cells (THP-1 cells or Jurkat cells). Analyses were performed based on the response of immune cells in conditioned media. Sprague-Dawley rats received WBI at different doses, and were fed for one week to one month postirradiation. Spleen and peripheral blood were then isolated and analyzed. We observed that the number of monocytes in peripheral blood, and the level of NK cells and NKT cells in spleen increased after CNS injury. However, the level of T cells in spleen did not change and the level of B cells in the spleen decreased after ?-ray-induced CNS injury. These findings indicate that CNS injury caused by ionizing radiation induces a series of changes in the peripheral immune system.
UR - http://www.scopus.com/inward/record.url?scp=85072992595&partnerID=8YFLogxK
U2 - 10.1667/RR15378.1
DO - 10.1667/RR15378.1
M3 - Article
C2 - 31393823
AN - SCOPUS:85072992595
SN - 0033-7587
VL - 192
SP - 440
EP - 450
JO - Radiation Research
JF - Radiation Research
IS - 4
ER -
Zhang P, Chen Y, Zhu H, Yan L, Sun C, Pei S 等. The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An in Vitro and in Vivo Study. Radiation Research. 2019 10月 1;192(4):440-450. doi: 10.1667/RR15378.1