The determination of the total flavonoids by UV and a flavone glycoside by HPLC in Torreya grandis Fort Leaves

Rongji Dai, Dan Jiang, Wei Li, Yongqian Zhang, Hong Yu, Yulin Deng*, Weiwei Meng

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

3 引用 (Scopus)

摘要

A UV method was established to determination the total flavonoids in extractive solution from Torreya grandis Fort, leaves, and a RP-HPLC method was developed and validated to quantify 4', 5, 7- Trihydroxyflavone- 5- O- [a- L- Rhamnopyranosyl- (1→;4)- 6- O- acetyl- β- D- glucopyranoside] (FRG). The dried leaf samples were extracted using methanol. The extracts were directly analyzed, without any purification, on a reversed-phase ODS column (250mm×4.6 mm, 5μm) with acetonitrile-phosphate aqueous (pH 3.8) isocratic elution and the detection was carried out by UV at 330 nm. The UV method showed excellent linearity, precision and accuracy. The standard curve for total flavonoids was linear over the range of 4-18μg·ml -1 (R2=0.9995). The HPLC method was validated for linearity, precision, accuracy, limit of detection and quantification. The standard curve for FRG was linear over the range of 0.39μ-3.53μg (R 2=0.9998). This method showed excellent repeatability (relative standard deviation, R.S.D.=2.0%), precision(R.S.D.=1.4%) and accuracy(102.27; R.S.D.=1.02%). The limits of detection (LOD) of FRG, based on a detector signal-to-noise ratio of 3, were 0.065mg ml-1 and the lowest levels tested in Torreya grandis Fort. leaves by this procedure (limit of quantification, LOQ) were 0.196 mg ml-1. This assay can be readily utilized as quality controlled method for Torreya grandis Fort preparations.

源语言英语
主期刊名2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
1887-1891
页数5
DOI
出版状态已出版 - 2007
活动2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, 中国
期限: 23 5月 200727 5月 2007

出版系列

姓名2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

会议

会议2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
国家/地区中国
Beijing
时期23/05/0727/05/07

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