TY - GEN
T1 - The determination of the total flavonoids by UV and a flavone glycoside by HPLC in Torreya grandis Fort Leaves
AU - Dai, Rongji
AU - Jiang, Dan
AU - Li, Wei
AU - Zhang, Yongqian
AU - Yu, Hong
AU - Deng, Yulin
AU - Meng, Weiwei
PY - 2007
Y1 - 2007
N2 - A UV method was established to determination the total flavonoids in extractive solution from Torreya grandis Fort, leaves, and a RP-HPLC method was developed and validated to quantify 4', 5, 7- Trihydroxyflavone- 5- O- [a- L- Rhamnopyranosyl- (1→;4)- 6- O- acetyl- β- D- glucopyranoside] (FRG). The dried leaf samples were extracted using methanol. The extracts were directly analyzed, without any purification, on a reversed-phase ODS column (250mm×4.6 mm, 5μm) with acetonitrile-phosphate aqueous (pH 3.8) isocratic elution and the detection was carried out by UV at 330 nm. The UV method showed excellent linearity, precision and accuracy. The standard curve for total flavonoids was linear over the range of 4-18μg·ml -1 (R2=0.9995). The HPLC method was validated for linearity, precision, accuracy, limit of detection and quantification. The standard curve for FRG was linear over the range of 0.39μ-3.53μg (R 2=0.9998). This method showed excellent repeatability (relative standard deviation, R.S.D.=2.0%), precision(R.S.D.=1.4%) and accuracy(102.27; R.S.D.=1.02%). The limits of detection (LOD) of FRG, based on a detector signal-to-noise ratio of 3, were 0.065mg ml-1 and the lowest levels tested in Torreya grandis Fort. leaves by this procedure (limit of quantification, LOQ) were 0.196 mg ml-1. This assay can be readily utilized as quality controlled method for Torreya grandis Fort preparations.
AB - A UV method was established to determination the total flavonoids in extractive solution from Torreya grandis Fort, leaves, and a RP-HPLC method was developed and validated to quantify 4', 5, 7- Trihydroxyflavone- 5- O- [a- L- Rhamnopyranosyl- (1→;4)- 6- O- acetyl- β- D- glucopyranoside] (FRG). The dried leaf samples were extracted using methanol. The extracts were directly analyzed, without any purification, on a reversed-phase ODS column (250mm×4.6 mm, 5μm) with acetonitrile-phosphate aqueous (pH 3.8) isocratic elution and the detection was carried out by UV at 330 nm. The UV method showed excellent linearity, precision and accuracy. The standard curve for total flavonoids was linear over the range of 4-18μg·ml -1 (R2=0.9995). The HPLC method was validated for linearity, precision, accuracy, limit of detection and quantification. The standard curve for FRG was linear over the range of 0.39μ-3.53μg (R 2=0.9998). This method showed excellent repeatability (relative standard deviation, R.S.D.=2.0%), precision(R.S.D.=1.4%) and accuracy(102.27; R.S.D.=1.02%). The limits of detection (LOD) of FRG, based on a detector signal-to-noise ratio of 3, were 0.065mg ml-1 and the lowest levels tested in Torreya grandis Fort. leaves by this procedure (limit of quantification, LOQ) were 0.196 mg ml-1. This assay can be readily utilized as quality controlled method for Torreya grandis Fort preparations.
KW - Determination
KW - FRG
KW - HPLC
KW - Total flavonoids
KW - UV
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=48149104034&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2007.4382076
DO - 10.1109/ICCME.2007.4382076
M3 - Conference contribution
AN - SCOPUS:48149104034
SN - 1424410789
SN - 9781424410781
T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
SP - 1887
EP - 1891
BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Y2 - 23 May 2007 through 27 May 2007
ER -