Synthesis of mesoporous silica material with hydrophobic external surface and hydrophilic internal surface for protein adsorption

Mesoporous silica materials have been increasingly used as immobilising and adsorptive carriers of proteins in the biochemical analysis field. The sustained requirements on protein adsorption carriers keep motivating chemists to seek new modified mesoporous silica materials to improve their protein adsorption capacity. With the aim to improve the material's capacity of absorbing protein, in this paper, we proposed a new approach to synthesise a modified mesoporous silica material sharing an external hydrophobic and internal hydrophilic surface (methyl-amino-SiO2). This was followed by the measurement of both the pore structure of the material and its capacity in absorbing hemoglobin. The experiment demonstrated that the new material maintained the original physical properties and showed a better absorptive capacity in protein. The better absorbability of the material comes from the novel synthesis method that chose the different chemical substitutes for replacing the exterior and inner surface of the material respectively. As the experimental results demonstrated, the modified mesoporous material supplied a better absorptive capacity in protein. As a result, it increased the applicability of the mesoporous material as adsorptive carriers of proteins in the biochemical analysis field.