Synthesis of chiral stationary phases via bonding β-cyclodextrin with α-schiff base and chromatographic performance

Two chiral stationary phases (BCDs and YBCDs) were prepared by bonding β-cyclodextrin derivative with α-Schiff base group from β-cyclodextrin monoaldehyde to the silica gel via 3-glyci-dyloxypropyltrimethoxysilane as a coupling agent. Using acetonitrile-TEAA as mobile phase, good enantiomeric resolutions were obtained for DL-aminoacide on BCDs and YBCDs, including Leucine, Threonine and Valine. It is also observed that the higher separation factor of threonine was at pH 7.11 for both of the column, and the optimal column temperatures of Leucine appeared at 30°C or 40°C, respectively for YBCDs and BCDs.