Jiang, B., Duan, D., Gao, L., Zhou, M., Fan, K., Tang, Y., Xi, J., Bi, Y., Tong, Z., Gao, G. F., Xie, N., Tang, A., Nie, G., Liang, M., & Yan, X. (2018). Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes. Nature Protocols, 13(7), 1506-1520. https://doi.org/10.1038/s41596-018-0001-1
Jiang, Bing ; Duan, Demin ; Gao, Lizeng 等. / Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes. 在: Nature Protocols. 2018 ; 卷 13, 号码 7. 页码 1506-1520.
@article{f2d40edb04fa41fdbb171034d82021db,
title = "Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes",
abstract = " Nanozymes are nanomaterials exhibiting intrinsic enzyme-like characteristics that have increasingly attracted attention, owing to their high catalytic activity, low cost and high stability. This combination of properties has enabled a broad spectrum of applications, ranging from biological detection assays to disease diagnosis and biomedicine development. Since the intrinsic peroxidase activity of Fe 3 O 4 nanoparticles (NPs) was first reported in 2007, >40 types of nanozymes have been reported that possess peroxidase-, oxidase-, haloperoxidase-or superoxide dismutase-like catalytic activities. Given the complex interdependence of the physicochemical properties and catalytic characteristics of nanozymes, it is important to establish a standard by which the catalytic activities and kinetics of various nanozymes can be quantitatively compared and that will benefit the development of nanozyme-based detection and diagnostic technologies. Here, we first present a protocol for measuring and defining the catalytic activity units and kinetics for peroxidase nanozymes, the most widely used type of nanozyme. In addition, we describe the detailed experimental procedures for a typical nanozyme strip-based biological detection test and demonstrate that nanozyme-based detection is repeatable and reliable when guided by the presented nanozyme catalytic standard. The catalytic activity and kinetics assays for a nanozyme can be performed within 4 h.",
author = "Bing Jiang and Demin Duan and Lizeng Gao and Mengjie Zhou and Kelong Fan and Yan Tang and Juqun Xi and Yuhai Bi and Zhou Tong and Gao, {George Fu} and Ni Xie and Aifa Tang and Guohui Nie and Minmin Liang and Xiyun Yan",
note = "Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = jul,
day = "1",
doi = "10.1038/s41596-018-0001-1",
language = "English",
volume = "13",
pages = "1506--1520",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Springer Nature",
number = "7",
}
Jiang, B, Duan, D, Gao, L, Zhou, M, Fan, K, Tang, Y, Xi, J, Bi, Y, Tong, Z, Gao, GF, Xie, N, Tang, A, Nie, G, Liang, M & Yan, X 2018, 'Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes', Nature Protocols, 卷 13, 号码 7, 页码 1506-1520. https://doi.org/10.1038/s41596-018-0001-1
Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes. /
Jiang, Bing; Duan, Demin; Gao, Lizeng 等.
在:
Nature Protocols, 卷 13, 号码 7, 01.07.2018, 页码 1506-1520.
科研成果: 期刊稿件 › 文章 › 同行评审
TY - JOUR
T1 - Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes
AU - Jiang, Bing
AU - Duan, Demin
AU - Gao, Lizeng
AU - Zhou, Mengjie
AU - Fan, Kelong
AU - Tang, Yan
AU - Xi, Juqun
AU - Bi, Yuhai
AU - Tong, Zhou
AU - Gao, George Fu
AU - Xie, Ni
AU - Tang, Aifa
AU - Nie, Guohui
AU - Liang, Minmin
AU - Yan, Xiyun
N1 - Publisher Copyright:
© 2018 The Author(s).
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Nanozymes are nanomaterials exhibiting intrinsic enzyme-like characteristics that have increasingly attracted attention, owing to their high catalytic activity, low cost and high stability. This combination of properties has enabled a broad spectrum of applications, ranging from biological detection assays to disease diagnosis and biomedicine development. Since the intrinsic peroxidase activity of Fe 3 O 4 nanoparticles (NPs) was first reported in 2007, >40 types of nanozymes have been reported that possess peroxidase-, oxidase-, haloperoxidase-or superoxide dismutase-like catalytic activities. Given the complex interdependence of the physicochemical properties and catalytic characteristics of nanozymes, it is important to establish a standard by which the catalytic activities and kinetics of various nanozymes can be quantitatively compared and that will benefit the development of nanozyme-based detection and diagnostic technologies. Here, we first present a protocol for measuring and defining the catalytic activity units and kinetics for peroxidase nanozymes, the most widely used type of nanozyme. In addition, we describe the detailed experimental procedures for a typical nanozyme strip-based biological detection test and demonstrate that nanozyme-based detection is repeatable and reliable when guided by the presented nanozyme catalytic standard. The catalytic activity and kinetics assays for a nanozyme can be performed within 4 h.
AB - Nanozymes are nanomaterials exhibiting intrinsic enzyme-like characteristics that have increasingly attracted attention, owing to their high catalytic activity, low cost and high stability. This combination of properties has enabled a broad spectrum of applications, ranging from biological detection assays to disease diagnosis and biomedicine development. Since the intrinsic peroxidase activity of Fe 3 O 4 nanoparticles (NPs) was first reported in 2007, >40 types of nanozymes have been reported that possess peroxidase-, oxidase-, haloperoxidase-or superoxide dismutase-like catalytic activities. Given the complex interdependence of the physicochemical properties and catalytic characteristics of nanozymes, it is important to establish a standard by which the catalytic activities and kinetics of various nanozymes can be quantitatively compared and that will benefit the development of nanozyme-based detection and diagnostic technologies. Here, we first present a protocol for measuring and defining the catalytic activity units and kinetics for peroxidase nanozymes, the most widely used type of nanozyme. In addition, we describe the detailed experimental procedures for a typical nanozyme strip-based biological detection test and demonstrate that nanozyme-based detection is repeatable and reliable when guided by the presented nanozyme catalytic standard. The catalytic activity and kinetics assays for a nanozyme can be performed within 4 h.
UR - http://www.scopus.com/inward/record.url?scp=85049589365&partnerID=8YFLogxK
U2 - 10.1038/s41596-018-0001-1
DO - 10.1038/s41596-018-0001-1
M3 - Article
C2 - 29967547
AN - SCOPUS:85049589365
SN - 1754-2189
VL - 13
SP - 1506
EP - 1520
JO - Nature Protocols
JF - Nature Protocols
IS - 7
ER -
Jiang B, Duan D, Gao L, Zhou M, Fan K, Tang Y 等. Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes. Nature Protocols. 2018 7月 1;13(7):1506-1520. doi: 10.1038/s41596-018-0001-1