Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon

Chuangui Sheng, Jian Zhao, Zhenghan Di, Yuanyu Huang, Yuliang Zhao, Lele Li*

*此作品的通讯作者

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摘要

The in vivo optical imaging of RNA biomarkers of inflammation is hindered by low signal-to-background ratios, owing to non-specific signal amplification in healthy tissues. Here we report the design and in vivo applicability, for the imaging of inflammation-associated messenger RNAs (mRNAs), of a molecular beacon bearing apurinic/apyrimidinic sites, whose amplification of fluorescence is triggered by human apurinic/apyrimidinic endonuclease 1 on translocation from the nucleus into the cytoplasm specifically in inflammatory cells. We assessed the sensitivity and tissue specificity of an engineered molecular beacon targeting interleukin-6 (IL-6) mRNA in live mice, by detecting acute inflammation in their paws and drug-induced inflammation in their livers. This enzymatic-amplification strategy may enable the specific and sensitive imaging of other disease-relevant RNAs in vivo.

源语言英语
页(从-至)1074-1084
页数11
期刊Nature Biomedical Engineering
6
9
DOI
出版状态已出版 - 9月 2022

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Sheng, C., Zhao, J., Di, Z., Huang, Y., Zhao, Y., & Li, L. (2022). Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon. Nature Biomedical Engineering, 6(9), 1074-1084. https://doi.org/10.1038/s41551-022-00932-z