@inbook{a78b92c9a812432490350277e478530f,
title = "siRNA design and galNAc-empowered hepatic targeted delivery",
abstract = "Small interfering RNA (siRNA) is a clinically approved therapeutic modality, which has attracted widespread attention not only from basic research but also from pharmaceutical industry. As siRNA can theoretically modulate any disease-related gene{\textquoteright}s expression, plenty of siRNA therapeutic pipelines have been established by tens of biotechnology companies. The drug performance of siRNA heavily depends on the sequence, the chemical modification, and the delivery of siRNA. Here, we describe the rational design protocol of siRNA, and provide some modification patterns that can enhance siRNA{\textquoteright}s stability and reduce its off-target effect. Also, the delivery method based on N-acetylgalactosamine (GalNAc)-siRNA conjugate that is widely employed to develop therapeutic regimens for liver-related diseases is also recapitulated.",
keywords = "GalNAc, Nucleic acid therapeutic, RNA interference, RNAi, siRNA delivery, siRNA design, siRNA modification",
author = "Mei Lu and Mengjie Zhang and Bo Hu and Yuanyu Huang",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-1298-9_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "77--100",
booktitle = "Methods in Molecular Biology",
}