TY - JOUR
T1 - Single-Shot Through-Focus Image Acquisition and Phase Retrieval From Chromatic Aberration and Multi-Angle Illumination
AU - Zhou, Guocheng
AU - Zhang, Shaohui
AU - Zhai, Yayu
AU - Hu, Yao
AU - Hao, Qun
N1 - Publisher Copyright:
© Copyright © 2021 Zhou, Zhang, Zhai, Hu and Hao.
PY - 2021/4/29
Y1 - 2021/4/29
N2 - Phase recovery from a stack of through-focus intensity images is an effective non-interference quantitative phase imaging strategy. Nevertheless, the implementations of these methods are expensive and time-consuming because the distance between each through-focus plane has to be guaranteed by precision mechanical moving devices, and the multiple images must be acquired sequentially. In this article, we propose a single-shot through-focus intensity image stack acquisition strategy without any precision movement. Isolated LED units are used to illuminate the sample in different colors from different angles. Due to the chromatic aberration characteristics of the objective, the color-channel defocus images on the theoretical imaging plane are mutually laterally shifted. By calculating the shift amount of each sub-image area in each color channel, the distances between each through-focus image can be obtained, which is a critical parameter in transport of intensity equation (TIE) and alternating projection (AP). Lastly, AP is used to recover the phase distribution and realize the 3D localization of different defocus distances of the sample under test as an example. Both simulation and experiments are conducted to verify the feasibility of the proposed method.
AB - Phase recovery from a stack of through-focus intensity images is an effective non-interference quantitative phase imaging strategy. Nevertheless, the implementations of these methods are expensive and time-consuming because the distance between each through-focus plane has to be guaranteed by precision mechanical moving devices, and the multiple images must be acquired sequentially. In this article, we propose a single-shot through-focus intensity image stack acquisition strategy without any precision movement. Isolated LED units are used to illuminate the sample in different colors from different angles. Due to the chromatic aberration characteristics of the objective, the color-channel defocus images on the theoretical imaging plane are mutually laterally shifted. By calculating the shift amount of each sub-image area in each color channel, the distances between each through-focus image can be obtained, which is a critical parameter in transport of intensity equation (TIE) and alternating projection (AP). Lastly, AP is used to recover the phase distribution and realize the 3D localization of different defocus distances of the sample under test as an example. Both simulation and experiments are conducted to verify the feasibility of the proposed method.
KW - alternating projection
KW - chromatic aberration
KW - computational imaging
KW - microscopy
KW - phase retrieval
KW - single-shot
UR - http://www.scopus.com/inward/record.url?scp=85105921352&partnerID=8YFLogxK
U2 - 10.3389/fphy.2021.648827
DO - 10.3389/fphy.2021.648827
M3 - Article
AN - SCOPUS:85105921352
SN - 2296-424X
VL - 9
JO - Frontiers in Physics
JF - Frontiers in Physics
M1 - 648827
ER -