TY - JOUR
T1 - Simultaneous detection of Alzheimer's biomarkers using a visual electrochemiluminescence bipolar array
AU - Jia, Yi Lei
AU - Li, Xiao Qiong
AU - Chen, Hong Yuan
AU - Zhao, Wei
AU - Xu, Jing Juan
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Alzheimer's disease (AD), a progressive neurological disorder, presents challenges in early detection due to the limitations and costs of neuropsychological tests and neuroimaging. This study introduces a visual electrochemiluminescence (ECL) sensing device employing red and green signal lights on dual-channel closed bipolar electrodes (BPEs) for simultaneous Aβ oligomers and Tau proteins detection. To mitigate cross-chemical reactions, we physically separated target detection and ECL production within different chambers connected by BPEs. Utilizing efficient gold clusters (Au NCs) and Ru(bpy)32+ as green and red emitters, respectively, along with methylene blue (MB) as the recognition probe on the cathode sensing platform, we achieved a detection limit of 2.6 pM for Aβ oligomers and 0.3 pM for Tau proteins by measuring visual ECL intensity at two anodic poles of BPEs. This approach minimizes cross-chemical reactions, ensures robust ECL signal sensors, and allows integration of multiple sensors on a single chip. The proposed portable bipolar electrode chips employing ECL technology show promise for early and precise Alzheimer's disease diagnosis and personal healthcare.
AB - Alzheimer's disease (AD), a progressive neurological disorder, presents challenges in early detection due to the limitations and costs of neuropsychological tests and neuroimaging. This study introduces a visual electrochemiluminescence (ECL) sensing device employing red and green signal lights on dual-channel closed bipolar electrodes (BPEs) for simultaneous Aβ oligomers and Tau proteins detection. To mitigate cross-chemical reactions, we physically separated target detection and ECL production within different chambers connected by BPEs. Utilizing efficient gold clusters (Au NCs) and Ru(bpy)32+ as green and red emitters, respectively, along with methylene blue (MB) as the recognition probe on the cathode sensing platform, we achieved a detection limit of 2.6 pM for Aβ oligomers and 0.3 pM for Tau proteins by measuring visual ECL intensity at two anodic poles of BPEs. This approach minimizes cross-chemical reactions, ensures robust ECL signal sensors, and allows integration of multiple sensors on a single chip. The proposed portable bipolar electrode chips employing ECL technology show promise for early and precise Alzheimer's disease diagnosis and personal healthcare.
KW - Alzheimer's disease
KW - Electrochemiluminescence
KW - Simultaneous detection
KW - Visualized ECL-BPE device
UR - http://www.scopus.com/inward/record.url?scp=85171327138&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2023.134591
DO - 10.1016/j.snb.2023.134591
M3 - Article
AN - SCOPUS:85171327138
SN - 0925-4005
VL - 396
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 134591
ER -