TY - GEN
T1 - Separation of 2-heptamine by HPLC used cyclodextrin as chiral selector
AU - Dai, Rongji
AU - Liu, Wei
AU - Yang, Lin
AU - Deng, Yulin
PY - 2007
Y1 - 2007
N2 - HFLC was used to separate chiral compouds with chiral column. Another method was used in HFLC with chiral mobile phase additives to separate chiral compounds [1-3]. Using chiral mobile phase additives is a good way to separate the chiral confounds. The structure of the derivative takes an important role in separation.We derivatized 2-heptamine with benzene sulfonyl chloride and dansyl chloride, Using cyclodextrin derivatives as chiral mobile phase additives, 2-heptamine derivatives was separated by HPLC. 2-heptamine derivatives have a good absorb stance under 254nm. We separated these derivative with chiral mobile phase additives, the additives were β-CD, 2-O-(2,3-dihydroxypropyl)-β-CD, 2-β-(hydroxypropyl)-β-CD and so on. The additives are all highly hydro-soluble and good for separation. We used phosphoric buffer solution to supply proper pH range and ion intensity. A good separation result was obtained using 2-O-(hydrosypropyl)-β-CD. It could be used in separation of molecule hike aliphatic amine. Benzene sulfonyl chloride and dansyl chloride could be used to derivatize 2-heptamine for its chiral separation. And we got some conclusion about how to separate alkyl amine, and how to select the chiral mobile phase additives. We got a mechanism that the role chiral mobile phase additives played during the separation.
AB - HFLC was used to separate chiral compouds with chiral column. Another method was used in HFLC with chiral mobile phase additives to separate chiral compounds [1-3]. Using chiral mobile phase additives is a good way to separate the chiral confounds. The structure of the derivative takes an important role in separation.We derivatized 2-heptamine with benzene sulfonyl chloride and dansyl chloride, Using cyclodextrin derivatives as chiral mobile phase additives, 2-heptamine derivatives was separated by HPLC. 2-heptamine derivatives have a good absorb stance under 254nm. We separated these derivative with chiral mobile phase additives, the additives were β-CD, 2-O-(2,3-dihydroxypropyl)-β-CD, 2-β-(hydroxypropyl)-β-CD and so on. The additives are all highly hydro-soluble and good for separation. We used phosphoric buffer solution to supply proper pH range and ion intensity. A good separation result was obtained using 2-O-(hydrosypropyl)-β-CD. It could be used in separation of molecule hike aliphatic amine. Benzene sulfonyl chloride and dansyl chloride could be used to derivatize 2-heptamine for its chiral separation. And we got some conclusion about how to separate alkyl amine, and how to select the chiral mobile phase additives. We got a mechanism that the role chiral mobile phase additives played during the separation.
KW - 2-heptamine
KW - Chiral separation
KW - Cyclodextrin
KW - HPLC
UR - http://www.scopus.com/inward/record.url?scp=48149091385&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2007.4382073
DO - 10.1109/ICCME.2007.4382073
M3 - Conference contribution
AN - SCOPUS:48149091385
SN - 1424410789
SN - 9781424410781
T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
SP - 1875
EP - 1878
BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Y2 - 23 May 2007 through 27 May 2007
ER -