Separation cis-trans isomers and enantiomers of sertraline hydrochloride by capillary zone electrophoresis

Aim: To study the factors influencing the separation of cis-trans isomers and enantiomers of sertraline hydrochloride, particularly the influences of different types of cyclodextrins (CDs) on the chiral selectivity, and to establish the method of for the separation of cis-trans isomers and enantiomers of sertraline hydrochloride by capillary zone electrophoresis (CZE). Methods: Optimal separation was achieved using a buffer (pH 11) of 30 mmol/L sodium borate containing 15 mg/mL CM-β-CD; The voltage and temperature was 15 kV and 20 °C, respectively; The wavelength of 210 nm was used for detection. Results: Baseline resolution of cis-trans isomers and enantiomers of sertraline hydrochloride was reached in 15 run min. Conclusion: The chiral selectivity of negatively charged CDs is better than that of the neutral CDs.