Selection and identification of H1N1 influenza virus hemagglutinin inhibitory peptides

Objective Using the influenza virus hemagglutinin (HA) as the target to screen for novel anti-influenza polypeptide drugs. Methods The HA binding peptides were screened out through affinity selection from a 12-peptide phage library, and the anti-H1N1 activity was evaluated at MDCK cell and chicken embryo(ovo). Results Nine HA binding peptides were finally obtained, and the H6 peptide was found having significant antiviral activity against H1N1. Its IC₅₀ against two strains of H1N1, A/FM1/1/47 (H1N1) and A/PPR8/34(H 1 N 1), were 37. 3 and 48. 5 μmoZL respectively determined by cytopathic effect (CPE)test, and 26. 7 and 33. 4 μmoI/L respectively measured by ovo antiviral experiment. Conclusion These results showed that H6 might be a potential herapeu icdrug for H1N1 infecion.