Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

Jun Mei Wang; Yuan Shan Zeng; Ran Yi Liu; Wen Lin Huang; Yi Xiong; Yan Hua Wang; Shui Jun Chen; Yang D. Teng

doi:10.1016/j.expneurol.2006.07.028

Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

Jun Mei Wang, Yuan Shan Zeng^*, Ran Yi Liu, Wen Lin Huang, Yi Xiong, Yan Hua Wang, Shui Jun Chen, Yang D. Teng

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30 引用（Scopus）

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We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P < 0.05, χ² test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.

源语言	英语
页（从-至）	123-127
页数	5
期刊	Experimental Neurology
卷	203
期	1
DOI	https://doi.org/10.1016/j.expneurol.2006.07.028
出版状态	已出版 - 1月 2007
已对外发布	是

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@article{3339297db9e043d99f92d9f41fb9855d,

title = "Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells",

abstract = "We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P < 0.05, χ2 test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.",

keywords = "Differentiation, Gene expression, Neural stem cell, NT-3, Recombinant adenovirus, TrkC",

author = "Wang, {Jun Mei} and Zeng, {Yuan Shan} and Liu, {Ran Yi} and Huang, {Wen Lin} and Yi Xiong and Wang, {Yan Hua} and Chen, {Shui Jun} and Teng, {Yang D.}",

year = "2007",

month = jan,

doi = "10.1016/j.expneurol.2006.07.028",

language = "English",

volume = "203",

pages = "123--127",

journal = "Experimental Neurology",

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T1 - Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

AU - Wang, Jun Mei

AU - Zeng, Yuan Shan

AU - Liu, Ran Yi

AU - Huang, Wen Lin

AU - Xiong, Yi

AU - Wang, Yan Hua

AU - Chen, Shui Jun

AU - Teng, Yang D.

PY - 2007/1

Y1 - 2007/1

N2 - We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P < 0.05, χ2 test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.

AB - We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P < 0.05, χ2 test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.

KW - Differentiation

KW - Gene expression

KW - Neural stem cell

KW - NT-3

KW - Recombinant adenovirus

KW - TrkC

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U2 - 10.1016/j.expneurol.2006.07.028

DO - 10.1016/j.expneurol.2006.07.028

M3 - Article

C2 - 17007838

AN - SCOPUS:33751430281

SN - 0014-4886

VL - 203

SP - 123

EP - 127

JO - Experimental Neurology

JF - Experimental Neurology

IS - 1

ER -

Recombinant adenovirus vector-mediated functional expression of neurotropin-3 receptor (TrkC) in neural stem cells

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