Preliminary study on the preparation of enzyme reactor using the method of dynamic immobilization

Shanshan Wang*, Hua Li, Qin Li, Lina Geng, Feng Qu, Yulin Deng

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

摘要

In this study, an immobilized enzyme reactor was prepared in capillary by bio-functionalized chromatography method using immobilized artificial membrane as matrix and trypsin as immobilized enzyme. Bovine serum albumin, myoglobin, and cytochrome C were enzymolysised by the immobilized enzyme reactor and detected by HPLC-MS/MS techniques. The preliminary results demonstrated that the activity, stability, and catalytic efficiency of the immobilized enzyme reactor were high. Moreover, its catalytic activity can be retained for a long time and the stationary phase can be reused.

源语言英语
页(从-至)477-480
页数4
期刊Chemistry Bulletin / Huaxue Tongbao
71
6
出版状态已出版 - 2008

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