摘要
In this study, an immobilized enzyme reactor was prepared in capillary by bio-functionalized chromatography method using immobilized artificial membrane as matrix and trypsin as immobilized enzyme. Bovine serum albumin, myoglobin, and cytochrome C were enzymolysised by the immobilized enzyme reactor and detected by HPLC-MS/MS techniques. The preliminary results demonstrated that the activity, stability, and catalytic efficiency of the immobilized enzyme reactor were high. Moreover, its catalytic activity can be retained for a long time and the stationary phase can be reused.
源语言 | 英语 |
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页(从-至) | 477-480 |
页数 | 4 |
期刊 | Chemistry Bulletin / Huaxue Tongbao |
卷 | 71 |
期 | 6 |
出版状态 | 已出版 - 2008 |