Notice of Retraction: Characterization of salsolinol synthase from rat brain

Xuechai Chen, Abida Arshad, Yi Chen, Shengping Dai, Shengyuan Xiao, Yulin Deng*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节章节同行评审

摘要

Several studies estimating the function of salsolinol (1-methyl-6,7- dihydroxy-1,2,3,4-tetrahydroisoquinoline), a dopamine-derived endogenously synthesized compound, have revealed its involvement in the pathogenesis of neurodegenerative diseases such as Parkinson's disease (PD). Moreover, a number of investigations propose that N-methylated derivates of salsolinol, N-methyl-salsolinol and its metabolite products are also involved in etiopathogenesis of PD. The human brain has a higher concentration of (R) enantiomer of salsolinol than (S)enantiomer, suggesting its enzymatic biosynthesis. Recent studies in our lab have shown salsolinol synthesized from dopamine and acetaldehyde by a novel enzyme called salsolinol synthase. In current investigations, the brains of male Sprague Dawley rats were used for crude extract isolation and HPLC-electrochemical detection (ECD) was used for the activity assay of this putative enzyme. Our results show the enzyme activity of salsolinol synthase to be maximum at 80 °C and at pH of 7. The results also showed that amount of Salsolinol synthesized by salsolinol synthase is directly proportional to the amount of substrates dopamine (DA) and acetaldehyde (Ach) at 37 °C. Its isoelectric point was 8.0 determined with Off-gel. Further characterization of this putative enzyme is under process which will highlight more profound knowledge about this putative enzyme and hence, will help elucidate the pathogenesis of Parkinson's like diseases and will provide a strong basis for treatment of such neurodegenerative diseases.

源语言英语
主期刊名5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
出版商IEEE Computer Society
ISBN(印刷版)9781424450893
DOI
出版状态已出版 - 2011

出版系列

姓名5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

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Chen, X., Arshad, A., Chen, Y., Dai, S., Xiao, S., & Deng, Y. (2011). Notice of Retraction: Characterization of salsolinol synthase from rat brain. 在 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 文章 5780301 (5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011). IEEE Computer Society. https://doi.org/10.1109/icbbe.2011.5780301