Nanogapped Fe3O4@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Jiaxuan Li, Ting Wu, Chaoguang Wang, Jian Tu, Xiangjun Song, Ying Shao, Chongwen Wang*, Kezong Qi*, Rui Xiao*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

26 引用 (Scopus)

摘要

Herein, we present an ultrasensitive surface-enhanced Raman scattering (SERS)-based immunochromatographic assay (ICA) for the multiplex detection of foodborne bacteria in food samples by using nanogapped Fe3O4@Au (Mag@Au) as multifunctional SERS tags. The Mag@Au tags with multiple surface hotspots were integrated into the ICA system for the first time to provide a superior SERS signal for ICA analysis, high stability for complex samples, and magnetic enrichment ability for target pathogens. Visible test lines were successfully introduced by mixing bovine serum albumin (BSA)-coated gold nanoparticles (Au@BSA) with detection antibodies, which can facilitate the accurate positioning of test zones for quantitative SERS analysis. The magnetic SERS-ICA can achieve simultaneous and quantitative detection of Salmonella typhimurium and Staphylococcus aureus in 30 min with low limits of detection of 12 and 9 cells mL-1, respectively. The proposed method further demonstrated good stability and accuracy in various food samples and thus possesses great potential for on-site and sensitive monitoring of bacteria.

源语言英语
页(从-至)12679-12689
页数11
期刊ACS Applied Nano Materials
5
9
DOI
出版状态已出版 - 23 9月 2022
已对外发布

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