TY - GEN
T1 - Nano-flow multidimensional liquid platform using strong ion-exchange and reversed-phase chromatography for improved proteomic analyses
AU - Wang, Yun
AU - Liu, Yahui
AU - Lu, Song
AU - Li, Yujuan
AU - Zhang, Yongqian
AU - Qing, Hong
AU - Deng, Yulin
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/9/2
Y1 - 2015/9/2
N2 - In this study, an integrated multidimensional nano-flow liquid chromatography platform was established with the combination of peptide separation via online SCX, and successfully applied for proteome analysis. By this platform, peptides were first separated by a SCX microcolumn under a series of salt steps, separated by nano-reversed phase liquid chromatography, and finally identified by ESI-MS/MS. To evaluate the performance, the rat hippocampal cytosol proteins were analyzed. Ten fractions were loaded onto a SCX column via autosampler and analyzed by LC-MS/MS. More than 3323 spectra matched to peptides, and 3141 unique peptide were searched. A total of 600 proteins were identified in this single analysis. Compared with the results obtained by offline 2D-Nano-RPLC-MS/MS approach, the identified protein number was increased upto 13%, with decreased consumed time from 96 to 18 h. These results shows that such an integrated approach would gain attention for better proteome results.
AB - In this study, an integrated multidimensional nano-flow liquid chromatography platform was established with the combination of peptide separation via online SCX, and successfully applied for proteome analysis. By this platform, peptides were first separated by a SCX microcolumn under a series of salt steps, separated by nano-reversed phase liquid chromatography, and finally identified by ESI-MS/MS. To evaluate the performance, the rat hippocampal cytosol proteins were analyzed. Ten fractions were loaded onto a SCX column via autosampler and analyzed by LC-MS/MS. More than 3323 spectra matched to peptides, and 3141 unique peptide were searched. A total of 600 proteins were identified in this single analysis. Compared with the results obtained by offline 2D-Nano-RPLC-MS/MS approach, the identified protein number was increased upto 13%, with decreased consumed time from 96 to 18 h. These results shows that such an integrated approach would gain attention for better proteome results.
KW - Nano-flow LC
KW - Proteome
KW - hippocampus cytosol
KW - integrated platform
KW - offline 2D chromatography
UR - http://www.scopus.com/inward/record.url?scp=84955242944&partnerID=8YFLogxK
U2 - 10.1109/ICMA.2015.7237582
DO - 10.1109/ICMA.2015.7237582
M3 - Conference contribution
AN - SCOPUS:84955242944
T3 - 2015 IEEE International Conference on Mechatronics and Automation, ICMA 2015
SP - 767
EP - 771
BT - 2015 IEEE International Conference on Mechatronics and Automation, ICMA 2015
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 12th IEEE International Conference on Mechatronics and Automation, ICMA 2015
Y2 - 2 August 2015 through 5 August 2015
ER -