TY - JOUR
T1 - Microchip-Grafted P(NIPAAm-co-VPBA) with Thermoresponsive Boronate Affinity for Capture–Release of cis-Diol Biomolecules
AU - Man, Yan
AU - Peng, Guang
AU - Lv, Xuefei
AU - Liang, Yanli
AU - Wang, Yun
AU - Chen, Ye
AU - Deng, Yulin
N1 - Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.
PY - 2014/2
Y1 - 2014/2
N2 - Based on the successful graft of P(N-isopropylacrylamide-4-vinylphenylboronic acid) [P(NIPAAm-co-VPBA)] on glass substrates by surface-initiated atom transfer radical polymerization, a microfludic chip with the performance of thermoresponsive boronate affinity was fabricated and was used for capture–release of the cis-diol biomolecule adenosine. The copolymers modified successfully on the surface of glass substrate were characterized by Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results demonstrated that, by simply adjusting the temperature from 4 to 55 °C, the microchip grafted with P(NIPAAm-co-VPBA) was specific to the cis-diol-containing molecules, and can selectively and quickly realize the capture and release of the cis-diol biomolecule adenosine from the mixture of adenosine and deoxyadenosine, which was validated by high performance liquid chromatography/electronic spray ion–ion trap mass spectrometry (HPLC/ESI–ion trap MS). Moreover, less than 10 min was taken from injection to the collection of the sample at the end of the channel. With further development, the P(NIPAAm-co-VPBA)-grafted microchip with high selectivity and repeatability may be chosen as a promising tool for the enrichment of the cis-diol-containing biomolecules, such as glycoproteins, carbohydrates, glycosylated peptides, nucleosides, etc.
AB - Based on the successful graft of P(N-isopropylacrylamide-4-vinylphenylboronic acid) [P(NIPAAm-co-VPBA)] on glass substrates by surface-initiated atom transfer radical polymerization, a microfludic chip with the performance of thermoresponsive boronate affinity was fabricated and was used for capture–release of the cis-diol biomolecule adenosine. The copolymers modified successfully on the surface of glass substrate were characterized by Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results demonstrated that, by simply adjusting the temperature from 4 to 55 °C, the microchip grafted with P(NIPAAm-co-VPBA) was specific to the cis-diol-containing molecules, and can selectively and quickly realize the capture and release of the cis-diol biomolecule adenosine from the mixture of adenosine and deoxyadenosine, which was validated by high performance liquid chromatography/electronic spray ion–ion trap mass spectrometry (HPLC/ESI–ion trap MS). Moreover, less than 10 min was taken from injection to the collection of the sample at the end of the channel. With further development, the P(NIPAAm-co-VPBA)-grafted microchip with high selectivity and repeatability may be chosen as a promising tool for the enrichment of the cis-diol-containing biomolecules, such as glycoproteins, carbohydrates, glycosylated peptides, nucleosides, etc.
KW - 4-Vinylphenylboronic acid (VPBA)
KW - Boronate affinity
KW - Poly(N-isopropylacrylamide) (PNIPAAm)
KW - Thermoresponsive
KW - cis-Diol
UR - http://www.scopus.com/inward/record.url?scp=84925491019&partnerID=8YFLogxK
U2 - 10.1007/s10337-014-2821-7
DO - 10.1007/s10337-014-2821-7
M3 - Article
AN - SCOPUS:84925491019
SN - 0009-5893
VL - 78
SP - 157
EP - 162
JO - Chromatographia
JF - Chromatographia
IS - 3-4
ER -