TY - GEN
T1 - Micro-well array based genetic determination of EGFR mutation at single-cell level
AU - Li, Ren
AU - Zhou, Mingxing
AU - Li, Jine
AU - Zhang, Weikai
AU - Wang, Zihua
AU - Wei, Zewen
AU - Hu, Zhiyuan
PY - 2016
Y1 - 2016
N2 - We developed a double-sided micro-well array microfluidic chip which consists of three parts: microfluidic channel, cell trapping array and cell lysate collecting chamber. We integrated single cell trapping, identification and in situ cell lysis in this microfluidic chip to differentiate Epidermal Growth Factor Receptor (EGFR)-expressed cancer cells from normal cells, for detecting not only the existence of EGFR expression, but also various mutation types at single cell level.
AB - We developed a double-sided micro-well array microfluidic chip which consists of three parts: microfluidic channel, cell trapping array and cell lysate collecting chamber. We integrated single cell trapping, identification and in situ cell lysis in this microfluidic chip to differentiate Epidermal Growth Factor Receptor (EGFR)-expressed cancer cells from normal cells, for detecting not only the existence of EGFR expression, but also various mutation types at single cell level.
KW - Epidermal growth factor receptor mutation
KW - Genetic analysis
KW - Single cell analysis
UR - http://www.scopus.com/inward/record.url?scp=85014188560&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014188560
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 206
EP - 207
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -
