TY - JOUR
T1 - Magnetic quantum dot based lateral flow assay biosensor for multiplex and sensitive detection of protein toxins in food samples
AU - Wang, Chongwen
AU - Xiao, Rui
AU - Wang, Shu
AU - Yang, Xingsheng
AU - Bai, Zikun
AU - Li, Xinying
AU - Rong, Zhen
AU - Shen, Beifen
AU - Wang, Shengqi
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/12/15
Y1 - 2019/12/15
N2 - Protein toxins, such as botulinum neurotoxin type A (BoNT/A) and staphylococcal enterotoxin B (SEB), easily pollute food and water and are ultra-toxic to humans and animals, thus requiring a sensitive on-site detection method. In this study, we reported a novel lateral flow assay (LFA) strip on the basis of magnetic quantum dot nanoparticles (MagQD NPs) for sensitive and multiplex protein toxin detection in food samples. A new type of MagQD NP was prepared by fixing the dense carboxylated QDs on the surface of polyethyleneimine-modified Fe3O4 magnetic NPs (MNPs) and applied in LFA with the following functions: capture and enrich target toxins from sample solutions and serve as advanced fluorescent labels for the quantitative determination of targets on the strip. Through this strategy, the assay realized quantified BoNT/A and SEB detection in 30 min with the limits of detection of 2.52 and 2.86 pg/mL, respectively. The selectivity and the ability of quantitative analysis of the method were validated in real food samples, including milk and juice. This MagQD-LFA biosensor showed considerable potential as a point-of-care testing tool for the sensitive detection of trace toxins.
AB - Protein toxins, such as botulinum neurotoxin type A (BoNT/A) and staphylococcal enterotoxin B (SEB), easily pollute food and water and are ultra-toxic to humans and animals, thus requiring a sensitive on-site detection method. In this study, we reported a novel lateral flow assay (LFA) strip on the basis of magnetic quantum dot nanoparticles (MagQD NPs) for sensitive and multiplex protein toxin detection in food samples. A new type of MagQD NP was prepared by fixing the dense carboxylated QDs on the surface of polyethyleneimine-modified Fe3O4 magnetic NPs (MNPs) and applied in LFA with the following functions: capture and enrich target toxins from sample solutions and serve as advanced fluorescent labels for the quantitative determination of targets on the strip. Through this strategy, the assay realized quantified BoNT/A and SEB detection in 30 min with the limits of detection of 2.52 and 2.86 pg/mL, respectively. The selectivity and the ability of quantitative analysis of the method were validated in real food samples, including milk and juice. This MagQD-LFA biosensor showed considerable potential as a point-of-care testing tool for the sensitive detection of trace toxins.
KW - Botulinum neurotoxin type A
KW - Fluorescent lateral flow immunoassay
KW - Magnetic-quantum dot nanoparticles
KW - Simultaneous detection
KW - Staphylococcal enterotoxin
UR - http://www.scopus.com/inward/record.url?scp=85072921785&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2019.111754
DO - 10.1016/j.bios.2019.111754
M3 - Article
C2 - 31605985
AN - SCOPUS:85072921785
SN - 0956-5663
VL - 146
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 111754
ER -