TY - JOUR
T1 - Magnetic Nanotag-Based Colorimetric/SERS Dual-Readout Immunochromatography for Ultrasensitive Detection of Clenbuterol Hydrochloride and Ractopamine in Food Samples
AU - Wu, Ting
AU - Li, Jiaxuan
AU - Zheng, Shuai
AU - Yu, Qing
AU - Qi, Kezong
AU - Shao, Ying
AU - Wang, Chongwen
AU - Tu, Jian
AU - Xiao, Rui
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/9
Y1 - 2022/9
N2 - Direct and sensitive detection of multiple illegal additives in complex food samples is still a challenge in on-site detection. In this study, an ultrasensitive immunochromatographic assay (ICA) using magnetic Fe3O4@Au nanotags as a capture/detection difunctional tool was developed for the direct detection of β2-adrenoceptor agonists in real samples. The Fe3O4@Au tag is composed of a large magnetic core (~160 nm), a rough Au nanoshell, dense surface-modified Raman molecules, and antibodies, which cannot only effectively enrich targets from complex solutions to reduce the matrix effects of food samples and improve detection sensitivity, but also provide strong colorimetric/surface-enhanced Raman scattering (SERS) dual signals for ICA testing. The dual readout signals of the proposed ICA can meet the detection requirements in different environments. Specifically, the colorimetric signal allows for rapid visual detection of the analyte, and the SERS signal is used for the sensitive and quantitative detection modes. The proposed dual-signal ICA can achieve the simultaneous determination of two illegal additives, namely, clenbuterol hydrochloride and ractopamine. The detection limits for the two targets via colorimetric and SERS signals were down to ng mL−1 and pg mL−1 levels, respectively. Moreover, the proposed assay has demonstrated high accuracy and stability in real food samples.
AB - Direct and sensitive detection of multiple illegal additives in complex food samples is still a challenge in on-site detection. In this study, an ultrasensitive immunochromatographic assay (ICA) using magnetic Fe3O4@Au nanotags as a capture/detection difunctional tool was developed for the direct detection of β2-adrenoceptor agonists in real samples. The Fe3O4@Au tag is composed of a large magnetic core (~160 nm), a rough Au nanoshell, dense surface-modified Raman molecules, and antibodies, which cannot only effectively enrich targets from complex solutions to reduce the matrix effects of food samples and improve detection sensitivity, but also provide strong colorimetric/surface-enhanced Raman scattering (SERS) dual signals for ICA testing. The dual readout signals of the proposed ICA can meet the detection requirements in different environments. Specifically, the colorimetric signal allows for rapid visual detection of the analyte, and the SERS signal is used for the sensitive and quantitative detection modes. The proposed dual-signal ICA can achieve the simultaneous determination of two illegal additives, namely, clenbuterol hydrochloride and ractopamine. The detection limits for the two targets via colorimetric and SERS signals were down to ng mL−1 and pg mL−1 levels, respectively. Moreover, the proposed assay has demonstrated high accuracy and stability in real food samples.
KW - dual-readout
KW - immunochromatographic assay
KW - magnetic SERS tags
KW - simultaneous detection
KW - β2-adrenoceptor agonists
UR - http://www.scopus.com/inward/record.url?scp=85138743564&partnerID=8YFLogxK
U2 - 10.3390/bios12090709
DO - 10.3390/bios12090709
M3 - Article
AN - SCOPUS:85138743564
SN - 2079-6374
VL - 12
JO - Biosensors
JF - Biosensors
IS - 9
M1 - 709
ER -