Kinetics of horseradish peroxidase-catalyzed nitration of phenol in a biphasic system

Mingming Kong, Yang Zhang, Qida Li, Runan Dong, Haijun Gao*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

7 引用 (Scopus)

摘要

The use of peroxidase in the nitration of phenols is gaining interest as compared with traditional chemical reactions. We investigated the kinetic characteristics of phenol nitration catalyzed by horseradish peroxidase (HRP) in an aqueous-organic biphasic system using n-butanol as the organic solvent and NO2- and H2O2 as substrates. The reaction rate was mainly controlled by the reaction kinetics in the aqueous phase when appropriate agitation was used to enhance mass transfer in the biphasic system. The initial velocity of the reaction increased with increasing HRP concentration. Additionally, an increase in the substrate concentrations of phenol (0–2 mM in organic phase) or H2O2 (0–0.1 mM in aqueous phase) enhanced the nitration efficiency catalyzed by HRP. In contrast, high concentrations of organic solvent decreased the kinetic parameter Vmax/Km. No inhibition of enzyme activity was observed when the concentrations of phenol and H2O2 were at or below 10 mM and 0.1 mM, respectively. On the basis of the peroxidase catalytic mechanism, a double-substrate ping-pong kinetic model was established. The kinetic parameters were KmH2O2 = 1.09 mM, KmPhOH = 9.45 mM, and Vmax = 0.196 mM/min. The proposed model was well fit to the data obtained from additional independent experiments under the suggested optimal synthesis conditions. The kinetic model developed in this paper lays a foundation for further comprehensive study of enzymatic nitration kinetics.

源语言英语
页(从-至)297-305
页数9
期刊Journal of Microbiology and Biotechnology
27
2
DOI
出版状态已出版 - 2月 2017

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Kong, M., Zhang, Y., Li, Q., Dong, R., & Gao, H. (2017). Kinetics of horseradish peroxidase-catalyzed nitration of phenol in a biphasic system. Journal of Microbiology and Biotechnology, 27(2), 297-305. https://doi.org/10.4014/jmb.1607.07039