Interacting effects of tributyltin and 17β-estradiol in male Chinese loach (Misgurnus anguillicaudatus)

In this study, male Chinese loaches in a semistatic waterborne exposure system were used to study the effects of tributyltin (TBT) on vitellogenin (Vtg) production induced by 17β-estradiol (E2), TBT accumulation and distribution in tissues, and the effects of E2 on the distribution of TBT. The results demonstrate that TBT does not induce the synthesis of Vtg in male Chinese loach and that TBT could significantly inhibit Vtg production induced by E2, after exposure to binary mixtures of E2 and TBT for 14 days. TBT was found to accumulate in the liver, kidney, gills, intestines, and muscles of male Chinese loach, wherein the liver, kidney, gills, and intestines were found to have the most TBT accumulation. The existence of E2 did not significantly affect tissue distribution of TBT.