Immobilization of formate dehydrogenase on polyethyleneimine modified carriers for the enhancement of catalytic performance

Polyethyleneimine (PEI) is a common modifier in the field of CO₂ adsorption but rarely used in the immobilization of enzyme catalyzing CO₂ conversion. Here, formate dehydrogenase (FDH) was immobilized on the surface of PEI modified polyethylene membrane or silica microspheres and then applied in the reaction of CO₂ to formate. After optimizing the immobilization conditions, the catalytic performance of immobilized FDH was respectively 4.3 and 7.4 times of free enzyme attributed to the promotion effect of the carrier. Polydopamine/PEI-SiO₂ attached FDH exhibited a higher activity and stability than biocatalytic membrane.