Imaging of Gut Bacterial Macroscopic Changes in Simulated Microgravity-Exposed Rats via In Vivo Metabolic Labeling

Zhujun Wu, Huayan Liu, Liben Yan, Yifan Deng, Zhongqin Tian, Yiyang Du, Yuankun Zhao, Hong Ma, Yulin Deng, Yujuan Li*, Zhimin Wang*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

摘要

The impact of the microgravity environment on gut bacteria has been widely recognized to induce notable gastrointestinal pathology during extended spaceflight. However, most current studies for gut microbiome homeostasis profiling are based on the 16S rRNA gene sequencing of fecal samples; this technology faces challenges in analyzing gut bacterial alterations in situ, dynamically, and with high spatiotemporal resolution. Herein, we present the utilization of bioorthogonal metabolic labeling for noninvasive imaging of gut bacterial macroscopic changes in simulated microgravity (SMG) rats. After being subsequently labeled with the metabolic reporters d-Ala-N3 and ICG-DBCO through click chemistry, it was shown that SMG can trigger obvious perturbation of gut bacteria, evidenced by the significant increase in the total bacterial content and spatial distribution variations. Such a difference was accompanied by the occurrence of intestinal inflammation and tissue damage. Compared with 16S rRNA genome analysis focusing on composition and diversity, the metabolic labeling strategy provides unprecedented insights into the macroscopic changes of the gut bacterial content and distribution under SMG. Our study will be helpful for investigating the biological implication of SMG-induced imbalance in gut bacteria, potentially promoting the deep investigation of the complex gastrointestinal pathology in space biomedicine.

源语言英语
页(从-至)19758-19767
页数10
期刊Analytical Chemistry
96
49
DOI
出版状态已出版 - 10 12月 2024

指纹

探究 'Imaging of Gut Bacterial Macroscopic Changes in Simulated Microgravity-Exposed Rats via In Vivo Metabolic Labeling' 的科研主题。它们共同构成独一无二的指纹。

引用此

Wu, Z., Liu, H., Yan, L., Deng, Y., Tian, Z., Du, Y., Zhao, Y., Ma, H., Deng, Y., Li, Y., & Wang, Z. (2024). Imaging of Gut Bacterial Macroscopic Changes in Simulated Microgravity-Exposed Rats via In Vivo Metabolic Labeling. Analytical Chemistry, 96(49), 19758-19767. https://doi.org/10.1021/acs.analchem.4c05028