High throughput synthesis of oligonucleotide utilizing inkjet printer and micro-reactor array filled with robust opal

H. Li, Y. Huang, H. Q. Yu, Y. Ma, C. Y. Tang, Z. W. Wei, Z. C. Liang, W. Wang, Z. J. Yang, Z. H. Li*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

1 引用 (Scopus)

摘要

Oligonucleotide synthesis with high throughput is a current challenge in modern life science. This paper presents a high throughput oligonucleotide synthesizer based on inkjet printer and micro-reactor array chip. Integration of the micro-reactor array filled with robust opal and a modified commercial inkjet printer enables the synthesis of 100 different oligonucleotides on a 20mm×20mm chip. Utilizing the prototype system, we successfully synthesized oligonucleotides of 6 monomers at a yield of about 100 pmol. This oligonucleotide synthesizer can be potentially used for genomic applications, which require tons of probes and primers in a short time.

源语言英语
主期刊名17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版商Chemical and Biological Microsystems Society
1242-1244
页数3
ISBN(印刷版)9781632666246
出版状态已出版 - 2013
已对外发布
活动17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, 德国
期限: 27 10月 201331 10月 2013

出版系列

姓名17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
2

会议

会议17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
国家/地区德国
Freiburg
时期27/10/1331/10/13

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