TY - JOUR
T1 - Graphene oxide-based three-dimensional Au nanofilm with high-density and controllable hotspots
T2 - A powerful film-type SERS tag for immunochromatographic analysis of multiple mycotoxins in complex samples
AU - Zheng, Shuai
AU - Wang, Chaoguang
AU - Li, Jiaxuan
AU - Wang, Wenqi
AU - Yu, Qing
AU - Wang, Chongwen
AU - Wang, Shengqi
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/11/15
Y1 - 2022/11/15
N2 - Co-contamination of mycotoxins in food and environment is a common phenomenon, which easily causes cumulative and synergistic damaging effects to human and animal health, poses great health and economy burdens to the world. A convenient technology for detection of multiple and low-concentration mycotoxins in real samples is highly desired but remains a challenge. Here, we proposed a multiplexed surface-enhanced Raman scattering (SERS)-immunochromatographic assay (ICA) that can sensitively and simultaneously detect three mycotoxins in unprocessed complex samples, using a graphene oxide-based three-dimensional (3D) Au nanofilm (called GO@Au-Au) as the film-type SERS tag. A precise sub-1 nm PEI layer was constructed into the GO@Au-Au nanostructure as built-in nanogap, which can accommodate Raman reporter molecules and create stable hotspots between the inner GO@Au film and outer assembled AuNP satellites. Grafting the 30 nm AuNPs onto the 2D GO@Au nanofilm greatly improved the SERS activity and colorimetric signal of film-type tags. Compared with traditional spherical SERS tags, the film-type GO@Au-Au can provide greater reaction interface, excellent stability and dispersibility and multiple SERS hotspots over large area for ICA using. Given these advantages, the proposed SERS-ICA can achieve simultaneous quantitative detection of fumonisin B1, aflatoxin B1, and zearalenone, with low detection limit (0.529, 0.745, and 5.90 pg mL−1), short testing time (20 min), and high accuracy for real food/experimental samples. Our method shows great potential to fulfill practical requirements for detection of multiple mycotoxins.
AB - Co-contamination of mycotoxins in food and environment is a common phenomenon, which easily causes cumulative and synergistic damaging effects to human and animal health, poses great health and economy burdens to the world. A convenient technology for detection of multiple and low-concentration mycotoxins in real samples is highly desired but remains a challenge. Here, we proposed a multiplexed surface-enhanced Raman scattering (SERS)-immunochromatographic assay (ICA) that can sensitively and simultaneously detect three mycotoxins in unprocessed complex samples, using a graphene oxide-based three-dimensional (3D) Au nanofilm (called GO@Au-Au) as the film-type SERS tag. A precise sub-1 nm PEI layer was constructed into the GO@Au-Au nanostructure as built-in nanogap, which can accommodate Raman reporter molecules and create stable hotspots between the inner GO@Au film and outer assembled AuNP satellites. Grafting the 30 nm AuNPs onto the 2D GO@Au nanofilm greatly improved the SERS activity and colorimetric signal of film-type tags. Compared with traditional spherical SERS tags, the film-type GO@Au-Au can provide greater reaction interface, excellent stability and dispersibility and multiple SERS hotspots over large area for ICA using. Given these advantages, the proposed SERS-ICA can achieve simultaneous quantitative detection of fumonisin B1, aflatoxin B1, and zearalenone, with low detection limit (0.529, 0.745, and 5.90 pg mL−1), short testing time (20 min), and high accuracy for real food/experimental samples. Our method shows great potential to fulfill practical requirements for detection of multiple mycotoxins.
KW - Built-in nanogap
KW - Graphene oxide-based Au nanofilm
KW - Multiplex detection
KW - Mycotoxin
KW - SERS-based immunochromatographic assay
UR - http://www.scopus.com/inward/record.url?scp=85132864857&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2022.137760
DO - 10.1016/j.cej.2022.137760
M3 - Article
AN - SCOPUS:85132864857
SN - 1385-8947
VL - 448
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
M1 - 137760
ER -