Graphene oxide-based three-dimensional Au nanofilm with high-density and controllable hotspots: A powerful film-type SERS tag for immunochromatographic analysis of multiple mycotoxins in complex samples

Shuai Zheng, Chaoguang Wang, Jiaxuan Li, Wenqi Wang, Qing Yu, Chongwen Wang*, Shengqi Wang

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

41 引用 (Scopus)

摘要

Co-contamination of mycotoxins in food and environment is a common phenomenon, which easily causes cumulative and synergistic damaging effects to human and animal health, poses great health and economy burdens to the world. A convenient technology for detection of multiple and low-concentration mycotoxins in real samples is highly desired but remains a challenge. Here, we proposed a multiplexed surface-enhanced Raman scattering (SERS)-immunochromatographic assay (ICA) that can sensitively and simultaneously detect three mycotoxins in unprocessed complex samples, using a graphene oxide-based three-dimensional (3D) Au nanofilm (called GO@Au-Au) as the film-type SERS tag. A precise sub-1 nm PEI layer was constructed into the GO@Au-Au nanostructure as built-in nanogap, which can accommodate Raman reporter molecules and create stable hotspots between the inner GO@Au film and outer assembled AuNP satellites. Grafting the 30 nm AuNPs onto the 2D GO@Au nanofilm greatly improved the SERS activity and colorimetric signal of film-type tags. Compared with traditional spherical SERS tags, the film-type GO@Au-Au can provide greater reaction interface, excellent stability and dispersibility and multiple SERS hotspots over large area for ICA using. Given these advantages, the proposed SERS-ICA can achieve simultaneous quantitative detection of fumonisin B1, aflatoxin B1, and zearalenone, with low detection limit (0.529, 0.745, and 5.90 pg mL−1), short testing time (20 min), and high accuracy for real food/experimental samples. Our method shows great potential to fulfill practical requirements for detection of multiple mycotoxins.

源语言英语
文章编号137760
期刊Chemical Engineering Journal
448
DOI
出版状态已出版 - 15 11月 2022
已对外发布

指纹

探究 'Graphene oxide-based three-dimensional Au nanofilm with high-density and controllable hotspots: A powerful film-type SERS tag for immunochromatographic analysis of multiple mycotoxins in complex samples' 的科研主题。它们共同构成独一无二的指纹。

引用此