TY - JOUR
T1 - Functions in rice endophyte pantoea agglomerans YS19
AU - Li, Qian Qian
AU - Xiong, Xiao Lu
AU - Mu, Yu Fei
AU - Yi, Ting
AU - Li, Er Li
AU - Lu, Zhen Yue
AU - Miao, Yu Xuan
AU - Feng, Yong Jun
PY - 2012
Y1 - 2012
N2 - Pantoea agglomerans YS19 is an endophytic diazotrophic bacterium isolated from rice (Oryza sativa cv. Yuefu) grown in temperate climatic regions in west Beijing (China). The bacterium forms aggregate structures called "symplasmata", in which several (at least two) to hundreds of individual cells tightly bind together. Our previous study revealed that there were two growth stages for YS19, including the single cell stage existing before exponential growth phase and the symplasmata forming stage starting at the end of the exponential growth phase in liquid LB medium. More strikingly, the symplasmata structures contribute to bacterial stress (e.g., dehydration, heavy metal toxicity, and osmotic shock) resistance and are especially significant for bacterial surviving strategy in suiting an adaptive life to hostile environments. In this research, YS19 was cultivated in LB medium, and the whole cellular protein expression of the cultures sampled at different times (0~12 h) was analyzed by SDS-PAGE. Here, a novel protein differentially expressed at the symplasmata forming stage was captured. The protein was purified and digested by trypsin. The digests were analyzed by MALDI-TOF mass spectrometry and the peptide mass fingerprint of this protein was successfully obtained. Then, database searching with Mascot in the SWISS-Prot database was performed using the recorded peptide mass fingerprint data. It is found that among 53 peptides, there are 14 peptide masses matching to MalE from Enterobacter aerogenes, which is a protein belonging to the periplasmic maltose-binding protein family of the ATP-binding cassette transporters. Then the gene of the MalE protein was cloned from YS19, expressed in the E. coli BL21 (DE3), and the recombinant protein was purified to homogeneity. Under stress (acid) treatments, the recombinant protein showed strong anti-aggregation ability, and even exhibited chaperone-like activity. This assay was achieved by comparing the quantity of the substrate proteins remaining in the supernatant under various denaturing acid conditions, where the aggregation of the substrate protein was effectively suppressed in the presence of the recombinant MalE protein. Far-UV circular dichroism spectroscopy examination on the purified MalE proteins under different pHs indicated that the global structures exhibited a sudden transition from a highly ordered conformation to a less ordered one. However, MalE was still capable of maintaining most of its secondary structures under these extreme acidic conditions (pH1~2). Finally, the Bis-ANS fluorescent probe technique was used to explore the effect of acid condition on the hydrophobic surfaces exposing of the protein. It is found that with the decrease of the pH value, the fluorescence intensity of the samples increased gradually, suggesting the MalE protein exposed the hydrophobic surfaces of the molecule to bind the substrates, implying the protein performed its chaperone activity via exposing its hydrophobic surfaces under acidic condition. MalE protein, originally found as a periplasmic maltose-binding protein of the ATP-binding cassette transporters, whose expression in YS19, however, far exceeds the amount being needed for transmembrane transports, is most likely a moonlighting protein for its alternative biological functions as a chaperone protein as revealed by this study. This research provided valuable information on the life styles and survival strategies of microorganisms that forms multicellular aggregates at specific growth stages and also explained the protein basis adopted by YS19 symplasmata-forming cells in surviving the stress conditions.
AB - Pantoea agglomerans YS19 is an endophytic diazotrophic bacterium isolated from rice (Oryza sativa cv. Yuefu) grown in temperate climatic regions in west Beijing (China). The bacterium forms aggregate structures called "symplasmata", in which several (at least two) to hundreds of individual cells tightly bind together. Our previous study revealed that there were two growth stages for YS19, including the single cell stage existing before exponential growth phase and the symplasmata forming stage starting at the end of the exponential growth phase in liquid LB medium. More strikingly, the symplasmata structures contribute to bacterial stress (e.g., dehydration, heavy metal toxicity, and osmotic shock) resistance and are especially significant for bacterial surviving strategy in suiting an adaptive life to hostile environments. In this research, YS19 was cultivated in LB medium, and the whole cellular protein expression of the cultures sampled at different times (0~12 h) was analyzed by SDS-PAGE. Here, a novel protein differentially expressed at the symplasmata forming stage was captured. The protein was purified and digested by trypsin. The digests were analyzed by MALDI-TOF mass spectrometry and the peptide mass fingerprint of this protein was successfully obtained. Then, database searching with Mascot in the SWISS-Prot database was performed using the recorded peptide mass fingerprint data. It is found that among 53 peptides, there are 14 peptide masses matching to MalE from Enterobacter aerogenes, which is a protein belonging to the periplasmic maltose-binding protein family of the ATP-binding cassette transporters. Then the gene of the MalE protein was cloned from YS19, expressed in the E. coli BL21 (DE3), and the recombinant protein was purified to homogeneity. Under stress (acid) treatments, the recombinant protein showed strong anti-aggregation ability, and even exhibited chaperone-like activity. This assay was achieved by comparing the quantity of the substrate proteins remaining in the supernatant under various denaturing acid conditions, where the aggregation of the substrate protein was effectively suppressed in the presence of the recombinant MalE protein. Far-UV circular dichroism spectroscopy examination on the purified MalE proteins under different pHs indicated that the global structures exhibited a sudden transition from a highly ordered conformation to a less ordered one. However, MalE was still capable of maintaining most of its secondary structures under these extreme acidic conditions (pH1~2). Finally, the Bis-ANS fluorescent probe technique was used to explore the effect of acid condition on the hydrophobic surfaces exposing of the protein. It is found that with the decrease of the pH value, the fluorescence intensity of the samples increased gradually, suggesting the MalE protein exposed the hydrophobic surfaces of the molecule to bind the substrates, implying the protein performed its chaperone activity via exposing its hydrophobic surfaces under acidic condition. MalE protein, originally found as a periplasmic maltose-binding protein of the ATP-binding cassette transporters, whose expression in YS19, however, far exceeds the amount being needed for transmembrane transports, is most likely a moonlighting protein for its alternative biological functions as a chaperone protein as revealed by this study. This research provided valuable information on the life styles and survival strategies of microorganisms that forms multicellular aggregates at specific growth stages and also explained the protein basis adopted by YS19 symplasmata-forming cells in surviving the stress conditions.
KW - Maltose-binding protein
KW - Moonlighting protein
KW - Pantoea agglomerans
KW - Rice endophyte
UR - http://www.scopus.com/inward/record.url?scp=84860844222&partnerID=8YFLogxK
U2 - 10.3724/SP.J.1206.2011.00259
DO - 10.3724/SP.J.1206.2011.00259
M3 - Article
AN - SCOPUS:84860844222
SN - 1000-3282
VL - 39
SP - 273
EP - 281
JO - Progress in Biochemistry and Biophysics
JF - Progress in Biochemistry and Biophysics
IS - 3
ER -