TY - JOUR
T1 - FRET effect on DNA fluorescent molecular probe by graphene oxide
AU - Zhang, Cong Xiao
AU - Lü, Xue Fei
AU - Qing, Hong
AU - Deng, Yu Lin
N1 - Publisher Copyright:
©, 2015, Beijing Institute of Technology. All right reserved.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Fluorescence resonance energy transfer (FRET) effect of graphene oxide on DNA fluorescent molecular probe is a very important factor of DNA the biosensor, which is related to the detection sensitivity of graphene oxide based DNA biosensors. The effects of the concentration of graphene oxide, the reaction temperature, and the length of DNA on FRET were investigated. The results show that the concentration of graphene oxide, the reaction temperature, and the length of DNA play significant roles on FRET effects. The fluorescence quenching efficiency of DNA molecule probe was improved with the increasing of graphene oxide concentration and the reaction temperature, with the decreasing of the length of DNA. Therefore, in order to get the excellent biosensor, the concentration of graphene oxide, the reaction temperature, and the length of DNA should be taken into account in the design of graphene oxide DNA biosensor.
AB - Fluorescence resonance energy transfer (FRET) effect of graphene oxide on DNA fluorescent molecular probe is a very important factor of DNA the biosensor, which is related to the detection sensitivity of graphene oxide based DNA biosensors. The effects of the concentration of graphene oxide, the reaction temperature, and the length of DNA on FRET were investigated. The results show that the concentration of graphene oxide, the reaction temperature, and the length of DNA play significant roles on FRET effects. The fluorescence quenching efficiency of DNA molecule probe was improved with the increasing of graphene oxide concentration and the reaction temperature, with the decreasing of the length of DNA. Therefore, in order to get the excellent biosensor, the concentration of graphene oxide, the reaction temperature, and the length of DNA should be taken into account in the design of graphene oxide DNA biosensor.
KW - Fluorescence resonance energy transfer (FRET)
KW - Graphene oxide
KW - Molecular probe
UR - http://www.scopus.com/inward/record.url?scp=84943400543&partnerID=8YFLogxK
U2 - 10.15918/j.tbit1001-0645.2015.supplement1.027
DO - 10.15918/j.tbit1001-0645.2015.supplement1.027
M3 - Article
AN - SCOPUS:84943400543
SN - 1001-0645
VL - 35
SP - 123
EP - 127
JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
ER -