Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

Mingshu Zhang; Zhifei Fu; Changqing Li; Anyuan Liu; Dingming Peng; Fudong Xue; Wenting He; Shan Gao; Fan Xu; Dan Xu; Ling Yuan; Fa Zhang; Zhiheng Xu; Tao Xu; Pingyong Xu

doi:10.1021/acs.nanolett.9b02855

Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

Mingshu Zhang, Zhifei Fu, Changqing Li, Anyuan Liu, Dingming Peng, Fudong Xue, Wenting He, Shan Gao, Fan Xu, Dan Xu, Ling Yuan, Fa Zhang, Zhiheng Xu, Tao Xu^*, Pingyong Xu

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20 引用（Scopus）

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Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm²-76 × 31.4 μm²) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.

源语言	英语
页（从-至）	2197-2208
页数	12
期刊	Nano Letters
卷	20
期	4
DOI	https://doi.org/10.1021/acs.nanolett.9b02855
出版状态	已出版 - 8 4月 2020
已对外发布	是

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title = "Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein",

abstract = "Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm2-76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific {"}parallel three-pillar{"} structure in the neuronal-glial cell junction, helping to elucidate glial cell {"}locking{"} and support of neurons during Drosophila embryogenesis.",

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author = "Mingshu Zhang and Zhifei Fu and Changqing Li and Anyuan Liu and Dingming Peng and Fudong Xue and Wenting He and Shan Gao and Fan Xu and Dan Xu and Ling Yuan and Fa Zhang and Zhiheng Xu and Tao Xu and Pingyong Xu",

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AU - Zhang, Mingshu

AU - Fu, Zhifei

AU - Li, Changqing

AU - Liu, Anyuan

AU - Peng, Dingming

AU - Xue, Fudong

AU - He, Wenting

AU - Gao, Shan

AU - Xu, Fan

AU - Xu, Dan

AU - Yuan, Ling

AU - Zhang, Fa

AU - Xu, Zhiheng

AU - Xu, Tao

AU - Xu, Pingyong

PY - 2020/4/8

Y1 - 2020/4/8

N2 - Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm2-76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.

AB - Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 × 9.4 μm2-76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.

KW - Drosophila embryos

KW - Photoconvertible fluorescent protein

KW - Quick-SIMBA

KW - live super-resolution microscopy

KW - pcStar

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Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

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