Extracting biomolecule collision cross sections from the high-resolution FT-ICR mass spectral linewidths

Ting Jiang, Yu Chen, Lu Mao, Alan G. Marshall*, Wei Xu

*此作品的通讯作者

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18 引用 (Scopus)

摘要

It is known that the ion collision cross section (CCS) may be calculated from the linewidth of a Fourier transform ion cyclotron resonance (FT-ICR) mass spectral peak at elevated pressure (e.g., ∼10-6 Torr). However, the high mass resolution of FT-ICR is sacrificed in those experiments due to high buffer gas pressure. In this study, we describe a linewidth correction method to eliminate the windowing-induced peak broadening effect. Together with the energetic ion-neutral collision model previously developed by our group, this method enables the extraction of CCSs of biomolecules from high-resolution FT-ICR mass spectral linewidths, obtained at a typical operating buffer gas pressure of modern FT-ICR instruments (∼10-10 Torr). CCS values of peptides including MRFA, angiotensin I, and bradykinin measured by the proposed method agree well with ion mobility measurements, and the unfolding of protein ions (ubiquitin) at higher charge states is also observed.

源语言英语
页(从-至)713-717
页数5
期刊Physical Chemistry Chemical Physics
18
2
DOI
出版状态已出版 - 2016

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