Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS

Mei Zhang; Ke Ming Shan; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.025

Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS

Mei Zhang, Ke Ming Shan, Yu Lin Deng

医学技术学院

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and ¹⁸O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.

源语言	英语
页（从-至）	116-118 and 127
期刊	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
卷	35
DOI	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.025
出版状态	已出版 - 1 7月 2015

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abstract = "An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.",

keywords = "Aging, Biomarker, HPLC-ESI TOF MS, Human serum albumin, O-labeling",

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doi = "10.15918/j.tbit1001-0645.2015.supplement1.025",

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AU - Zhang, Mei

AU - Shan, Ke Ming

AU - Deng, Yu Lin

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N2 - An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.

AB - An in vitro aging model was set up by methylglyoxal and human serum albumin based on maillard reaction according to cross-linking theory to evaluate aging degree individually. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify modification degree of peptides cleaved from HSA, which can indicate aging degree. The in vitro aging model was set up successfully, and all experimental conditions were optimized. This method was considered to be a good foundation for screening aging biomarkers in the future by quality evaluation of the optimized conditions.

KW - Aging

KW - Biomarker

KW - HPLC-ESI TOF MS

KW - Human serum albumin

KW - O-labeling

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SN - 1001-0645

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Establishment of peptide biomarker screening model in individual aging in vitro by HPLC/ESI-TOF MS

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