Electropositive Magnetic Fluorescent Nanoprobe-Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria

Jiaxuan Li, Zhengkang Li, Bingjie Wang, Qing Yu, Ting Wu, Chongwen Wang*, Bing Gu*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

摘要

Immunochromatographic assays (ICAs) provide simple and rapid strategies for bacterial diagnosis but still suffer from the problems of low sensitivity and high dependency on paired antibodies. Herein, the broad-spectrum capture and detection capability of the antibody-free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino-embedded SiO2 shell (MagMQD@Si+) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen. The superior performance of the MagMQD@Si+-based ICA through the multiplex detection of three common pathogens is demonstrated, namely, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Streptococcus typhi, showing that this ICA possesses high sensitivity (8–40 cells mL−1), good reproducibility (relative standard deviation < 5.4%), and high specificity for the three target bacteria. The clinical utility of the proposed method is verified through the detection of 30 real sputum samples from patients with bacterial respiratory infections, revealing that the MagMQD@Si+-based ICA has massive potential as a powerful inspection tool for the rapid, sensitive, and ultrasensitive diagnosis of bacterial infections.

源语言英语
期刊Advanced Science
DOI
出版状态已接受/待刊 - 2025
已对外发布

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Li, J., Li, Z., Wang, B., Yu, Q., Wu, T., Wang, C., & Gu, B. (已接受/印刷中). Electropositive Magnetic Fluorescent Nanoprobe-Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria. Advanced Science. https://doi.org/10.1002/advs.202412421