TY - GEN
T1 - Efficacy of low-power laser irradiation in the prevention of D-galactose induced senescence in human dermal fibroblasts
AU - Meng, Chengbo
AU - Wu, Shengnan
AU - Xing, Da
PY - 2011
Y1 - 2011
N2 - Low-power laser (He-Ne) irradiation (LPLI) has been found to modulate various biological effects, especially those involved in promoting cell proliferation and metabolic regulation. However, the underlying mechanisms that LPLI prevents human cell senescence remain undefined. Herein, we devised a model enabling cell senescence using D-galactose for two days then treat with or without LPLI(< 15J/cm2), and investigated whether LPLI delays cell senescent in human dermal fibroblasts cells (HDF-a). First in this study, using SA-β-gal staining, compared with control cell we detected a lower frequency of SA-β-gal staining under the treatment of LPLI. Moreover, we found the growth rates of cell with LPLI was higher using CCK-8 analysis. Additionally, we also found LPLI induced HDF-a entered the irreversible G1 arrest measured by flow cytometry system. Therefore, LPLI may promote cell proliferation by stimulating cell-cycle progression and delay human cell senescence. Taken together, Low-power laser irradiation delay HDF-a cells senescence provides new information for the mechanisms of biological effects of LPLI.
AB - Low-power laser (He-Ne) irradiation (LPLI) has been found to modulate various biological effects, especially those involved in promoting cell proliferation and metabolic regulation. However, the underlying mechanisms that LPLI prevents human cell senescence remain undefined. Herein, we devised a model enabling cell senescence using D-galactose for two days then treat with or without LPLI(< 15J/cm2), and investigated whether LPLI delays cell senescent in human dermal fibroblasts cells (HDF-a). First in this study, using SA-β-gal staining, compared with control cell we detected a lower frequency of SA-β-gal staining under the treatment of LPLI. Moreover, we found the growth rates of cell with LPLI was higher using CCK-8 analysis. Additionally, we also found LPLI induced HDF-a entered the irreversible G1 arrest measured by flow cytometry system. Therefore, LPLI may promote cell proliferation by stimulating cell-cycle progression and delay human cell senescence. Taken together, Low-power laser irradiation delay HDF-a cells senescence provides new information for the mechanisms of biological effects of LPLI.
KW - D-galactose
KW - Delay
KW - Low-power laser irradiation
KW - Senescence
UR - http://www.scopus.com/inward/record.url?scp=79955518397&partnerID=8YFLogxK
U2 - 10.1117/12.874155
DO - 10.1117/12.874155
M3 - Conference contribution
AN - SCOPUS:79955518397
SN - 9780819484246
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Mechanisms for Low-Light Therapy VI
T2 - Mechanisms for Low-Light Therapy VI
Y2 - 22 January 2011 through 23 January 2011
ER -