Effects of stem cell therapy on protein profile of parkinsonian rats using an18O-labeling quantitative proteomic approach

Yahui Liu; Kefu Liu; Wei Qin; Chenghao Liu; Xiaowei Zheng; Yulin Deng; Hong Qing

doi:10.1002/pmic.201500421

Effects of stem cell therapy on protein profile of parkinsonian rats using an¹⁸O-labeling quantitative proteomic approach

Yahui Liu, Kefu Liu, Wei Qin, Chenghao Liu, Xiaowei Zheng, Yulin Deng, Hong Qing^*

^*此作品的通讯作者

医学技术学院

Beijing Institute of Technology

科研成果: 期刊稿件 › 文章 › 同行评审

7 引用（Scopus）

摘要

The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with ¹⁸O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.

源语言	英语
页（从-至）	1023-1032
页数	10
期刊	Proteomics
卷	16
期	6
DOI	https://doi.org/10.1002/pmic.201500421
出版状态	已出版 - 1 3月 2016

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title = "Effects of stem cell therapy on protein profile of parkinsonian rats using an18O-labeling quantitative proteomic approach",

abstract = "The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with 18O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.",

keywords = "Animal proteomics, Neural stem cell, O-labeling, Parkinson's disease, Quantitative proteomics, Therapy",

author = "Yahui Liu and Kefu Liu and Wei Qin and Chenghao Liu and Xiaowei Zheng and Yulin Deng and Hong Qing",

note = "Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2016",

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AU - Liu, Yahui

AU - Liu, Kefu

AU - Qin, Wei

AU - Liu, Chenghao

AU - Zheng, Xiaowei

AU - Deng, Yulin

AU - Qing, Hong

PY - 2016/3/1

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N2 - The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with 18O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.

AB - The application of neural stem cell (NSC) research to neurodegenerative diseases has led to promising clinical trials. Currently, NSC therapy is most promising for Parkinson's disease (PD). We conducted behavioral tests and immunoassays for the profiling of a PD model in rats to assess the therapeutic effects of NSC treatments. Further, using a multiple sample comparison workflow, combined with 18O-labeled proteome mixtures, we compared the differentially expressed proteins from control, PD, and NSC-treated PD rats. The results were analyzed bioinformatically and verified by Western blot. Based on our initial findings, we believe that the proteomic approach is a valuable tool in evaluating the therapeutic effects of NSC transplantation on neurodegenerative disorders.

KW - Animal proteomics

KW - Neural stem cell

KW - O-labeling

KW - Parkinson's disease

KW - Quantitative proteomics

KW - Therapy

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Effects of stem cell therapy on protein profile of parkinsonian rats using an18O-labeling quantitative proteomic approach

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Effects of stem cell therapy on protein profile of parkinsonian rats using an¹⁸O-labeling quantitative proteomic approach