Development of an electrothermal MEMS mirror based two-photon microscopy probe

Liang Zhou, Yanpin Chen, Xiaohong Chen, Yunqi Hao, Jason E. Coleman, Huikai Xie

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

Two-photon microscopy (TPM) has shown its great impact in studying neuronal activity of live animals. Commercial TPM systems require the animals under study to be restrained under the microscope and the 3D images are obtained typically by using bulky galvo scanning mirrors, which limits the flexibility of animal brain imaging to a large extent. To study freely moving animals, miniaturization of the optical scanning system is the key. In this work, a miniature TPM probe has been developed based on an electrothermal MEMS mirror that can be driven under low voltage. The MEMS mirror has an initial tilt angle after fabrication, and its footprint is 3 mm x 4 mm and reflective mirror plate is aluminumcoated with an equivalent diameter of 2.5 mm. This MEMS probe can be directly adapted to a commercial TPM system. In addition to the MEMS mirror for laser beam scanning, inside the MEMS probe head there are a fixed mirror to fold the laser beam and a high NA polymer lens for focusing. This miniature probe can realize an FOV of 3.5°, or a scanning range of 150 μm. This MEMS probe head is compact with a size of 8 mm x 16 mm, which can be further scaled down.

源语言英语
主期刊名Multiphoton Microscopy in the Biomedical Sciences XIX
编辑Ammasi Periasamy, Peter T. C. So, Karsten Konig
出版商SPIE
ISBN(电子版)9781510624061
DOI
出版状态已出版 - 2019
活动Multiphoton Microscopy in the Biomedical Sciences XIX 2019 - San Francisco, 美国
期限: 3 2月 20196 2月 2019

出版系列

姓名Progress in Biomedical Optics and Imaging - Proceedings of SPIE
10882
ISSN(印刷版)1605-7422

会议

会议Multiphoton Microscopy in the Biomedical Sciences XIX 2019
国家/地区美国
San Francisco
时期3/02/196/02/19

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