Determination of ultrafiltration amount of different small intestinal mucosa in dogs with acute renal failure and the effects of ligustrazine

Aim: To observe and compare the effects of simple dialysate and different dose of ligustrazine dialysate on absorbability and ultrafiltration capacity of intestinal mucosa in dogs with acute renal failure (ARF), and analyze the effects of the actions of ligustrazine. Methods: The experiment was conducted in the Department of Clinical Laboratory, Central Hospital of Enshi Autonomous Prefecture and Experimental Center of Medical College of Hubei College for Nationalities between June and July 2004. 1Twenty female Beagle dogs were selected and randomly divided into 4 groups with 5 animals in each group: normal saline group, dialysate group, low dose ligustrazine group and high dose ligustrazine group. The jejunum, jejunoileum and ileum loop of dogs were isolated through operations according to the arteriovenous region of small intestine mesentery, so as to obstruct both renal arteries. The hypersonic sample solution was input through vein to establish model of ARF. From the beginning of sample-inputting, dogs in normal saline group, control group, low dose and high dose ligustrazine groups were input of normal saline, dialysate [dialysate (mmol/L): 180 of manicol, 60 of sodium, 3.7 of potassium, 46 of chlorine, 26.7 of bicarbonate, 1.6 of calcium, 35.1 of glucose and 7.34 of pH], dialysate containing 20mg/L ligustrazine and 205 mL of dialysate containing 80 mg/L ligustrazine from the proximate of each ansa interstinalis in the first 4 hours with the interval of 30 minutes. 3 mL of blood was drawn from the bole AtV of each intestinal mucosa, and the collection was accomplished within 6 hours with the total time of 360 minutes, and each sample was obtained for 13 times. The biochemical analytical apparatus and electrolytes analytical apparatus were adopted to detect the concentrations of K⁺,Na⁺, Cl^- in serum as well as area, creatinine and uric acid. 2 According to ¹³¹ I labelled albumen dilution method, the capacity of dialysate inside the ansa interstinalis was calculated. The absorbed dose of lymph was calculated by decreasing the plasma concentration of vein in given solute at given time-point from the plasma concentration of artery in given solute at given time. The total absorbed dose was equal to the plasma concentration of vein in given solute at given time plus the plasma concentration of artery in given solute at given time. Average ultrafiltration amount in intestinal mucosa at the 30th minute = (capacity of fluid in intestinal mucosa at the 6th hour-total input dialysate)/ 12.3Differences in measurement data among groups were compared with analysis of variance, and differences between each two ones were compared with Fisher's PLSD test. Results: A total of 20 dogs were involved in the analysis of results. 1 The secretory volume of jejunoileum: those in all dialysate groups at each time-point of experiment were obviously higher than the normal saline group (P < 0.01), and the apocenosis amount in low dose ligustrazine group at 30-240 minutes were significantly higher than the other three groups (P < 0.01). 2The absorbed dose of different segment of intestinal mucosa: the total absorbed dose in jejunum was the weakest, while that of lymph was the strongest, which was 5-8 times of other ansa interstinalis respectively (P < 0.01). The absorbed dose of lymph in each ansa interstinalis of high dose ligustrazine group were significantly lower than low dose ligustrazine group (P < 0.05), while those were higher than the normal saline group (P < 0.05-0.01). 3The average ultrafiltration amount at 30th minute: dialysate can remarkably enhance the average ultrafiltration amount of K⁺, Na⁺, Cl^-, urea, creatinine and uric acid (P < 0.05-0.01), and those of jejunum in each dialysate group were markedly the highest in all, which was in a decreasing trend from the jejunum to ileum loop. Compared with the same ansa interstinalis, that in low dose ligustrazine group was obviously higher than the other three groups (P < 0.01). Conclusion: The capacity of ultrafiltration and dialysate of small intestinal mucosa can be enhanced by adding low dose of ligustrazine into the dialysate, and the capacity of dialysate was the best in jejunum.