Determination of AKF-PD in whole blood of rat by HPLC-UV

Wei Cao; Lingyun He; Wenfang Liu; Zhizhuang Huang; Zeneng Cheng

doi:10.1016/j.jchromb.2006.01.010

Determination of AKF-PD in whole blood of rat by HPLC-UV

Wei Cao, Lingyun He, Wenfang Liu, Zhizhuang Huang, Zeneng Cheng^*

^*此作品的通讯作者

Central South University

科研成果: 期刊稿件 › 文章 › 同行评审

15 引用（Scopus）

摘要

An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C₁₈ column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml ^-1. The low limit of quantitation was 0.203 μg ml^-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.

源语言	英语
页（从-至）	257-259
页数	3
期刊	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
卷	833
期	2
DOI	https://doi.org/10.1016/j.jchromb.2006.01.010
出版状态	已出版 - 3 4月 2006
已对外发布	是

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title = "Determination of AKF-PD in whole blood of rat by HPLC-UV",

abstract = "An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C18 column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml -1. The low limit of quantitation was 0.203 μg ml-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.",

keywords = "AKF-PD, HPLC, Pharmacokinetics",

author = "Wei Cao and Lingyun He and Wenfang Liu and Zhizhuang Huang and Zeneng Cheng",

year = "2006",

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doi = "10.1016/j.jchromb.2006.01.010",

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T1 - Determination of AKF-PD in whole blood of rat by HPLC-UV

AU - Cao, Wei

AU - He, Lingyun

AU - Liu, Wenfang

AU - Huang, Zhizhuang

AU - Cheng, Zeneng

PY - 2006/4/3

Y1 - 2006/4/3

N2 - An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C18 column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml -1. The low limit of quantitation was 0.203 μg ml-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.

AB - An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C18 column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml -1. The low limit of quantitation was 0.203 μg ml-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.

KW - AKF-PD

KW - HPLC

KW - Pharmacokinetics

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DO - 10.1016/j.jchromb.2006.01.010

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AN - SCOPUS:33645218707

SN - 1570-0232

VL - 833

SP - 257

EP - 259

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Determination of AKF-PD in whole blood of rat by HPLC-UV

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