摘要
Electroporation is a high-efficiency and low-toxicity physical gene transfer method. Traditional electroporation is limited to only low volume cell samples. Here we present a continuous cell electroporation method based on commonly used microfluidic chip fabrication technology. Using easily fabricated PDMS microfluidic chip, syringe pumps, and pulse generator, we show efficient delivery of both DNA and siRNA into different cell lines. We describe the protocol of chip fabrication, apparatus setup, and cell electroporation assay. Typically, the fabrication of the devices takes 1 or 2 days and the continuous electroporation assay takes 1 h.
| 源语言 | 英语 |
|---|---|
| 主期刊名 | Electroporation Protocols |
| 主期刊副标题 | Preclinical and Clinical Gene Medicine |
| 出版商 | Humana Press Inc. |
| 页 | 99-110 |
| 页数 | 12 |
| ISBN（印刷版） | 9781461496311 |
| DOI | |
| 出版状态 | 已出版 - 2014 |
| 已对外发布 | 是 |
出版系列
| 姓名 | Methods in Molecular Biology |
|---|---|
| 卷 | 1121 |
| ISSN（印刷版） | 1064-3745 |
指纹
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Wei, Z., & Li, Z. (2014). Continuous cell electroporation for efficient DNA and siRNA delivery based on laminar microfluidic chips. 在 Electroporation Protocols: Preclinical and Clinical Gene Medicine (页码 99-110). (Methods in Molecular Biology; 卷 1121). Humana Press Inc.. https://doi.org/10.1007/978-1-4614-9632-8_8