TY - JOUR
T1 - Compact SchCas9 Recognizes the Simple NNGR PAM
AU - Wang, Shuai
AU - Mao, Huilin
AU - Hou, Linghui
AU - Hu, Ziying
AU - Wang, Yao
AU - Qi, Tao
AU - Tao, Chen
AU - Yang, Yuan
AU - Zhang, Chengdong
AU - Li, Miaomiao
AU - Liu, Huihui
AU - Hu, Shijun
AU - Chai, Renjie
AU - Wang, Yongming
N1 - Publisher Copyright:
© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH
PY - 2022/2/2
Y1 - 2022/2/2
N2 - Clustered regularly interspaced short palindromic repeat (CRISPR)/SaCas9 is the most popular tool for in vivo genome editing due to its high efficiency and small genome. The authors previously developed four SaCas9 orthologs as genome-editing tools. Here, to expand the targeting scope, they investigate the diversity of protospacer adjacent motifs (PAMs) by screening a list of 16 SaCas9 orthologs, twelve of which display editing activity in mammalian cells. They recognize five types of PAMs: NNGRRT, NNGRRR, NNGRC, NNGA, and NNGR. Importantly, SchCas9 recognizes the simple NNGR PAM, representing the most relaxed PAM preference of compact Cas9s to date. It is further demonstrated that SchCas9 enables efficient genome editing in multiple human cell lines. Altogether, these compact Cas9 tools offer a new option for both basic research and clinical applications.
AB - Clustered regularly interspaced short palindromic repeat (CRISPR)/SaCas9 is the most popular tool for in vivo genome editing due to its high efficiency and small genome. The authors previously developed four SaCas9 orthologs as genome-editing tools. Here, to expand the targeting scope, they investigate the diversity of protospacer adjacent motifs (PAMs) by screening a list of 16 SaCas9 orthologs, twelve of which display editing activity in mammalian cells. They recognize five types of PAMs: NNGRRT, NNGRRR, NNGRC, NNGA, and NNGR. Importantly, SchCas9 recognizes the simple NNGR PAM, representing the most relaxed PAM preference of compact Cas9s to date. It is further demonstrated that SchCas9 enables efficient genome editing in multiple human cell lines. Altogether, these compact Cas9 tools offer a new option for both basic research and clinical applications.
UR - http://www.scopus.com/inward/record.url?scp=85120542811&partnerID=8YFLogxK
U2 - 10.1002/advs.202104789
DO - 10.1002/advs.202104789
M3 - Article
C2 - 34874112
AN - SCOPUS:85120542811
SN - 2198-3844
VL - 9
JO - Advanced Science
JF - Advanced Science
IS - 4
M1 - 2104789
ER -