Chitinophaga solisilvae sp. Nov., isolated from forest soil

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, O9^T, was isolated from a forest soil sample collected at Dai, Xishuangbanna, Yunnan Province, PR China. Strain O9^T grew optimally at pH 7.0, at 28‒30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis placed strain O9^T within the genus Chitinophaga of the family Chitinophagaceae, with Chitinophaga terrae KP01^T (97.8%), Chitinophaga jiangningensis JN53^T (97.7%), Chitinophaga niastensis JS16-4^T (97.4%), Chitin-ophaga qingshengii JN246^T (97.3%) and Chitinophaga dinghuensis DHOC24^T (97.3%) as its closest relatives. Strain O9^T hydrolysed casein, gelatin and Tween 80. It could not assimilate l-arabinose, l-rhamnose, sucrose, melibiose, gentiobiose or d-fructose as a carbon source. It was negative for esterase lipase (C8) and β-glucosidase. Phosphatidylethanolamine was the predominant polar lipid. The major respiratory quinone of strain O9^T was MK-7. Its major fatty acids were iso-C_15:0 (34.2%), C_16:1 ω5c (20.9%) and iso-C_17:0 3-OH (12.6%). The genomic DNA G+C content of strain O9^T was 49.0 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain O9^T and that of Chitinophaga terrae KP01^T was 72.9%. The Genome-to-Genome Distance Calculator showed that DNA‒DNA hybridization values for strain O9^T and Chitinophaga terrae KP01^T were 13.6, 21.1 and 14.4%, respectively. Based on the polyphasic taxonomic data, strain O9^T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga solisilvae sp. nov. is proposed. The type strain is O9^T (=CGMCC 1.12462^T=KCTC 32404^T).