Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

Wen Tao Zhao; Zhen He Chen; Shu Yang; Xin Zhang; Li Quan Sun; Ai Qin Luo

doi:10.15918/j.tbit1001-0645.2015.supplement1.002

Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

Wen Tao Zhao, Zhen He Chen, Shu Yang, Xin Zhang, Li Quan Sun, Ai Qin Luo^*

^*此作品的通讯作者

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摘要

A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.

源语言	英语
页（从-至）	6-10
页数	5
期刊	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
卷	35
DOI	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.002
出版状态	已出版 - 1 7月 2015

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10.15918/j.tbit1001-0645.2015.supplement1.002

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title = "Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption",

abstract = "A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.",

keywords = "Fluorescence detection, Lysozyme, Molecularly imprinted polymer membranes, Polystyrene",

author = "Zhao, {Wen Tao} and Chen, {Zhen He} and Shu Yang and Xin Zhang and Sun, {Li Quan} and Luo, {Ai Qin}",

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T1 - Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

AU - Zhao, Wen Tao

AU - Chen, Zhen He

AU - Yang, Shu

AU - Zhang, Xin

AU - Sun, Li Quan

AU - Luo, Ai Qin

PY - 2015/7/1

Y1 - 2015/7/1

N2 - A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.

AB - A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.

KW - Fluorescence detection

KW - Lysozyme

KW - Molecularly imprinted polymer membranes

KW - Polystyrene

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SN - 1001-0645

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

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