Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle

Mengyao Tian, Jingyuan Huang, Hairong Xiao, Pei Jiang*, Xiangyu Ma, Yanqin Lin, Xujun Tang, Yintao Wang, Mingchen Dai, Wei Tong, Zixuan Ye, Xia Sheng, Renjie Chai, Shasha Zhang*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

摘要

Utricle is an important vestibular sensory organ for maintaining balance. 3,3′-iminodipropionitrile (IDPN), a prototype nitrile toxin, has been reported to be neurotoxic and vestibulotoxic, and can be used to establish an in vivo damage model of vestibular dysfunction. However, the mechanism of utricular HCs damage caused by IDPN is unclear. Here, we first studied mice balance behavior and HCs damage in IDPN utricle damage model, and found that IDPN injection in vivo can cause vestibular dysfunction and HCs damage, which is more pronounced than neomycin damage model. Then we used RNA-seq to characterize the transcriptome of IDPN damaged utricle in detail to identify genes and pathways that play roles in this process. We found 1,165 upregulated genes and 1,043 downregulated genes in IDPN damaged utricles, and identified that NF-κB pathway and TNF pathway may play important roles in IDPN damage model. Our study provides details of transcriptome of IDPN utricle damage model for further study of vestibular dysfunction.

源语言英语
文章编号1487364
期刊Frontiers in Molecular Neuroscience
17
DOI
出版状态已出版 - 2024
已对外发布

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Tian, M., Huang, J., Xiao, H., Jiang, P., Ma, X., Lin, Y., Tang, X., Wang, Y., Dai, M., Tong, W., Ye, Z., Sheng, X., Chai, R., & Zhang, S. (2024). Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle. Frontiers in Molecular Neuroscience, 17, 文章 1487364. https://doi.org/10.3389/fnmol.2024.1487364