TY - JOUR
T1 - Changes in p53 expression of spermatogenic cells in testis of aging rats after intervention with modified heshouwu pill
AU - Chen, Zhi Hong
AU - Gong, Xiu Yun
AU - Li, Jian
AU - Xue, Jing Feng
PY - 2007/4/29
Y1 - 2007/4/29
N2 - Aim: To observe the effects of modified heshouwu pill on spermatogenic cells apoptosis in testis, content of serum testosterone and anti-oxidation ability of aging rats. Methods: The experiment was carried out in the Institute of Basic Medical Sciences of Chengde Medical College from April to July 2004. 1 Forty male SD rats were selected and randomly divided into normal group, model group, modified heshouwu pill group and negative control group with 10 rats in each group. The rats except the normal group were intraperitoneally injected D-gal 200 mg/kg per day continually for 40 days to establish the sub-acute aging rat models. After modeling, no treatment was given to the rats in the model group; rats in the modified heshouwu pill group were intragastrically perfused of 8.1 g/kg modified heshouwu pill (consisted of 15 g tuber fleeceflower root, 15 g achyranthis root, 10 g desertliving cistanche, 10 g epimedium, 25 g danshen root and 15 g tuckahoe; each dose contained 90 g crude drug, and decocted into solution with water with traditional method), once a day for 30 days; and those in the negative control group were intragastrically perfused with saline of the same volume and at the same time. Rats in the normal group took food and drank freely and not received any intervention. 2 2 mL blood sample was drawn from the medial canthus of all rats, then the testosterone content in serum was detected by ELISA, the superoxide dismutase (SOD) activity in serum was detected by xanthine oxidase method. After the rats were killed, the testis tissue was taken out and embedded in paraffin; SP immunohistochemistry was used to observe the p53 expression of spermatogenic cells in testis. Results: All the 40 rats were involved in the result analysis. 1 Testosterone content in serum: The model group was obviously lower than the normal group (0.52±0.14), (1.26±0.32) μg/L, (t=3.004, P < 0.05); the content in the modified heshouwu pill group was (1.16±0.32) μg/L, which was higher than the model group (t=2.321, P < 0.05). 2 SOD activity in serum: The model group was lower than the normal group (106.22±9.63), (148.73±12.93) kNU/L, (t=13.339, P < 0.01); the activity in the modified heshouwu pill was (140.05±15.57) kNU/L, which was higher than the model group (t=9.970, P < 0.01). 3 p53 positive cell rate: The model group was significantly higher than the normal group (59.13±10.53)% and (36.99±9.45% X2 =9.696, P < 0.01); the rate of the modified heshouwu pill group was (43.03±12.65)%, which was lower than the model group (X2=5.122, P < 0.05). Conclusion: Traditional Chinese medicine modified heshouwu pill can improve the spermatogenic functions of aging rats, and has fairly anti-aging effects by improving the content of testosterone, enhancing the anti-oxidation ability and decreasing spermatogenic cells apoptosis.
AB - Aim: To observe the effects of modified heshouwu pill on spermatogenic cells apoptosis in testis, content of serum testosterone and anti-oxidation ability of aging rats. Methods: The experiment was carried out in the Institute of Basic Medical Sciences of Chengde Medical College from April to July 2004. 1 Forty male SD rats were selected and randomly divided into normal group, model group, modified heshouwu pill group and negative control group with 10 rats in each group. The rats except the normal group were intraperitoneally injected D-gal 200 mg/kg per day continually for 40 days to establish the sub-acute aging rat models. After modeling, no treatment was given to the rats in the model group; rats in the modified heshouwu pill group were intragastrically perfused of 8.1 g/kg modified heshouwu pill (consisted of 15 g tuber fleeceflower root, 15 g achyranthis root, 10 g desertliving cistanche, 10 g epimedium, 25 g danshen root and 15 g tuckahoe; each dose contained 90 g crude drug, and decocted into solution with water with traditional method), once a day for 30 days; and those in the negative control group were intragastrically perfused with saline of the same volume and at the same time. Rats in the normal group took food and drank freely and not received any intervention. 2 2 mL blood sample was drawn from the medial canthus of all rats, then the testosterone content in serum was detected by ELISA, the superoxide dismutase (SOD) activity in serum was detected by xanthine oxidase method. After the rats were killed, the testis tissue was taken out and embedded in paraffin; SP immunohistochemistry was used to observe the p53 expression of spermatogenic cells in testis. Results: All the 40 rats were involved in the result analysis. 1 Testosterone content in serum: The model group was obviously lower than the normal group (0.52±0.14), (1.26±0.32) μg/L, (t=3.004, P < 0.05); the content in the modified heshouwu pill group was (1.16±0.32) μg/L, which was higher than the model group (t=2.321, P < 0.05). 2 SOD activity in serum: The model group was lower than the normal group (106.22±9.63), (148.73±12.93) kNU/L, (t=13.339, P < 0.01); the activity in the modified heshouwu pill was (140.05±15.57) kNU/L, which was higher than the model group (t=9.970, P < 0.01). 3 p53 positive cell rate: The model group was significantly higher than the normal group (59.13±10.53)% and (36.99±9.45% X2 =9.696, P < 0.01); the rate of the modified heshouwu pill group was (43.03±12.65)%, which was lower than the model group (X2=5.122, P < 0.05). Conclusion: Traditional Chinese medicine modified heshouwu pill can improve the spermatogenic functions of aging rats, and has fairly anti-aging effects by improving the content of testosterone, enhancing the anti-oxidation ability and decreasing spermatogenic cells apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=34447254762&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:34447254762
SN - 1673-8225
VL - 11
SP - 3379
EP - 3381
JO - Chinese Journal of Tissue Engineering Research
JF - Chinese Journal of Tissue Engineering Research
IS - 17
ER -