Cell invasion in digital microfluidic microgel systems

Bingyu B. Li, Erica Y. Scott, M. Dean Chamberlain, Bill T.V. Duong, Shuailong Zhang, Susan J. Done, Aaron R. Wheeler*

*此作品的通讯作者

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25 引用 (Scopus)

摘要

Microfluidic methods for studying cell invasion can be subdivided into those in which cells invade into free space and those in which cells invade into hydrogels. The former techniques allow straightforward extraction of subpopulations of cells for RNA sequencing, while the latter preserve key aspects of cell interactions with the extracellular matrix (ECM). Here, we introduce “cell invasion in digital microfluidic microgel systems”(CIMMS), which bridges the gap between them, allowing the stratification of cells on the basis of their invasiveness into hydrogels for RNA sequencing. In initial studies with a breast cancer model, 244 genes were found to be differentially expressed between invading and noninvading cells, including genes correlating with ECM-remodeling, chemokine/ cytokine receptors, and G protein transducers. These results suggest that CIMMS will be a valuable tool for probing metastasis as well as the many physiological processes that rely on invasion, such as tissue development, repair, and protection.

源语言英语
文章编号eaba9589
期刊Science advances
6
29
DOI
出版状态已出版 - 7月 2020
已对外发布

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Li, B. B., Scott, E. Y., Dean Chamberlain, M., Duong, B. T. V., Zhang, S., Done, S. J., & Wheeler, A. R. (2020). Cell invasion in digital microfluidic microgel systems. Science advances, 6(29), 文章 eaba9589. https://doi.org/10.1126/sciadv.aba9589