Autonomic Integration in Nested Protocell Communities

Zhuping Yin, Ning Gao, Can Xu, Mei Li*, Stephen Mann*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

5 引用 (Scopus)

摘要

The self-driven organization of model protocells into higher-order nested cytomimetic systems with coordinated structural and functional relationships offers a step toward the autonomic implementation of artificial multicellularity. Here, we describe an endosymbiotic-like pathway in which proteinosomes are captured within membranized alginate/silk fibroin coacervate vesicles by guest-mediated reconfiguration of the host protocells. We demonstrate that interchange of coacervate vesicle and droplet morphologies through proteinosome-mediated urease/glucose oxidase activity produces discrete nested communities capable of integrated catalytic activity and selective disintegration. The self-driving capacity is modulated by an internalized fuel-driven process using starch hydrolases sequestered within the host coacervate phase, and structural stabilization of the integrated protocell populations can be achieved by on-site enzyme-mediated matrix reinforcement involving dipeptide supramolecular assembly or tyramine-alginate covalent cross-linking. Our work highlights a semi-autonomous mechanism for constructing symbiotic cell-like nested communities and provides opportunities for the development of reconfigurable cytomimetic materials with structural, functional, and organizational complexity.

源语言英语
页(从-至)14727-14736
页数10
期刊Journal of the American Chemical Society
145
27
DOI
出版状态已出版 - 12 7月 2023
已对外发布

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Yin, Z., Gao, N., Xu, C., Li, M., & Mann, S. (2023). Autonomic Integration in Nested Protocell Communities. Journal of the American Chemical Society, 145(27), 14727-14736. https://doi.org/10.1021/jacs.3c02816