Yang, M., Li, C., Ye, G., Shen, C., Shi, H., Zhong, L., Tian, Y., Zhao, M., Wu, P., Hussain, A., Zhang, T., Yang, H., Yang, J., Weng, Y., Liu, X., Wang, Z., Gan, L., Zhang, Q., Liu, Y., ... Zhao, Y. (2024). Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity. Signal Transduction and Targeted Therapy, 9(1), 文章 40. https://doi.org/10.1038/s41392-024-01748-w
Yang, Minghui ; Li, Chunhui ; Ye, Guoguo 等. / Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity. 在: Signal Transduction and Targeted Therapy. 2024 ; 卷 9, 号码 1.
@article{51b5d1543cf44e3aa37828367490f643,
title = "Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity",
abstract = "Emerging and recurrent infectious diseases caused by human coronaviruses (HCoVs) continue to pose a significant threat to global public health security. In light of this ongoing threat, the development of a broad-spectrum drug to combat HCoVs is an urgently priority. Herein, we report a series of anti-pan-coronavirus ssDNA aptamers screened using Systematic Evolution of Ligands by Exponential Enrichment (SELEX). These aptamers have nanomolar affinity with the nucleocapsid protein (NP) of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and also show excellent binding efficiency to the N proteins of both SARS, MERS, HCoV-OC43 and -NL63 with affinity KD values of 1.31 to 135.36 nM. Such aptamer-based therapeutics exhibited potent antiviral activity against both the authentic SARS-CoV-2 prototype strain and the Omicron variant (BA.5) with EC50 values at 2.00 nM and 41.08 nM, respectively. The protein docking analysis also evidenced that these aptamers exhibit strong affinities for N proteins of pan-coronavirus and other HCoVs (−229E and -HKU1). In conclusion, we have identified six aptamers with a high pan-coronavirus antiviral activity, which could potentially serve as an effective strategy for preventing infections by unknown coronaviruses and addressing the ongoing global health threat.",
author = "Minghui Yang and Chunhui Li and Guoguo Ye and Chenguang Shen and Huiping Shi and Liping Zhong and Yuxin Tian and Mengyuan Zhao and Pengfei Wu and Abid Hussain and Tian Zhang and Haiyin Yang and Jun Yang and Yuhua Weng and Xinyue Liu and Zhimin Wang and Lu Gan and Qianyu Zhang and Yingxia Liu and Ge Yang and Yuanyu Huang and Yongxiang Zhao",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",
year = "2024",
month = dec,
doi = "10.1038/s41392-024-01748-w",
language = "English",
volume = "9",
journal = "Signal Transduction and Targeted Therapy",
issn = "2095-9907",
publisher = "Springer Nature",
number = "1",
}
Yang, M, Li, C, Ye, G, Shen, C, Shi, H, Zhong, L, Tian, Y, Zhao, M, Wu, P, Hussain, A, Zhang, T, Yang, H, Yang, J, Weng, Y, Liu, X, Wang, Z, Gan, L, Zhang, Q, Liu, Y, Yang, G, Huang, Y & Zhao, Y 2024, 'Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity', Signal Transduction and Targeted Therapy, 卷 9, 号码 1, 40. https://doi.org/10.1038/s41392-024-01748-w
Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity. / Yang, Minghui
; Li, Chunhui; Ye, Guoguo 等.
在:
Signal Transduction and Targeted Therapy, 卷 9, 号码 1, 40, 12.2024.
科研成果: 期刊稿件 › 文章 › 同行评审
TY - JOUR
T1 - Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity
AU - Yang, Minghui
AU - Li, Chunhui
AU - Ye, Guoguo
AU - Shen, Chenguang
AU - Shi, Huiping
AU - Zhong, Liping
AU - Tian, Yuxin
AU - Zhao, Mengyuan
AU - Wu, Pengfei
AU - Hussain, Abid
AU - Zhang, Tian
AU - Yang, Haiyin
AU - Yang, Jun
AU - Weng, Yuhua
AU - Liu, Xinyue
AU - Wang, Zhimin
AU - Gan, Lu
AU - Zhang, Qianyu
AU - Liu, Yingxia
AU - Yang, Ge
AU - Huang, Yuanyu
AU - Zhao, Yongxiang
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12
Y1 - 2024/12
N2 - Emerging and recurrent infectious diseases caused by human coronaviruses (HCoVs) continue to pose a significant threat to global public health security. In light of this ongoing threat, the development of a broad-spectrum drug to combat HCoVs is an urgently priority. Herein, we report a series of anti-pan-coronavirus ssDNA aptamers screened using Systematic Evolution of Ligands by Exponential Enrichment (SELEX). These aptamers have nanomolar affinity with the nucleocapsid protein (NP) of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and also show excellent binding efficiency to the N proteins of both SARS, MERS, HCoV-OC43 and -NL63 with affinity KD values of 1.31 to 135.36 nM. Such aptamer-based therapeutics exhibited potent antiviral activity against both the authentic SARS-CoV-2 prototype strain and the Omicron variant (BA.5) with EC50 values at 2.00 nM and 41.08 nM, respectively. The protein docking analysis also evidenced that these aptamers exhibit strong affinities for N proteins of pan-coronavirus and other HCoVs (−229E and -HKU1). In conclusion, we have identified six aptamers with a high pan-coronavirus antiviral activity, which could potentially serve as an effective strategy for preventing infections by unknown coronaviruses and addressing the ongoing global health threat.
AB - Emerging and recurrent infectious diseases caused by human coronaviruses (HCoVs) continue to pose a significant threat to global public health security. In light of this ongoing threat, the development of a broad-spectrum drug to combat HCoVs is an urgently priority. Herein, we report a series of anti-pan-coronavirus ssDNA aptamers screened using Systematic Evolution of Ligands by Exponential Enrichment (SELEX). These aptamers have nanomolar affinity with the nucleocapsid protein (NP) of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and also show excellent binding efficiency to the N proteins of both SARS, MERS, HCoV-OC43 and -NL63 with affinity KD values of 1.31 to 135.36 nM. Such aptamer-based therapeutics exhibited potent antiviral activity against both the authentic SARS-CoV-2 prototype strain and the Omicron variant (BA.5) with EC50 values at 2.00 nM and 41.08 nM, respectively. The protein docking analysis also evidenced that these aptamers exhibit strong affinities for N proteins of pan-coronavirus and other HCoVs (−229E and -HKU1). In conclusion, we have identified six aptamers with a high pan-coronavirus antiviral activity, which could potentially serve as an effective strategy for preventing infections by unknown coronaviruses and addressing the ongoing global health threat.
UR - http://www.scopus.com/inward/record.url?scp=85185140838&partnerID=8YFLogxK
U2 - 10.1038/s41392-024-01748-w
DO - 10.1038/s41392-024-01748-w
M3 - Article
C2 - 38355661
AN - SCOPUS:85185140838
SN - 2095-9907
VL - 9
JO - Signal Transduction and Targeted Therapy
JF - Signal Transduction and Targeted Therapy
IS - 1
M1 - 40
ER -
Yang M, Li C, Ye G, Shen C, Shi H, Zhong L 等. Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity. Signal Transduction and Targeted Therapy. 2024 12月;9(1):40. doi: 10.1038/s41392-024-01748-w